Screening for lacI insert

Aim

The aim of this experiment is to screen for the lacI insert. This could be done using PCR but that would require specific primers and is not as reliable.

Protocol

• 1. Miniprep - the miniprep protocol was followed.
  2. Restriction digests - the restriction digests protocol was followed to allow the length of the DNA to be checked using single and double digest.
     • Single digest with Pst1
     • double digests with Pst1 and Xba1
  3. Gel electrophoresis - the gel electrophoresis protocol was followed.

Inference

• Set up liquid broth culture in LB