Team:Newcastle/16 July 2010

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{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
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*Screening for lacI insert:
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= Screening for lacI insert =
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*#Miniprep - The [[Team:Newcastle/Qiagen_Minipreps|miniprep]] protocol was followed.  
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*#Restriction digests
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== Aim ==
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The aim of this part of this experiment is to screen for the lacI insert.
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== Protocol ==
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*#Miniprep - the [[Team:Newcastle/Qiagen_Minipreps|miniprep]] protocol was followed.  
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*#Restriction digests - the [[Team:Newcastle/Restriction_digests|restriction digests]] protocol was followed to allow the length of the DNA to be checked using single and double digest.
*#*Single digest with Pst1  
*#*Single digest with Pst1  
*#*double digests with Pst1 and Xba1
*#*double digests with Pst1 and Xba1
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*#Gel electrophoresis
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*#Gel electrophoresis - the [[Team:Newcastle/Gel_electrophoresis|gel electrophoresis]] protocol was followed.
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Inference
*Set up liquid broth culture in LB
*Set up liquid broth culture in LB
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Revision as of 10:50, 5 August 2010

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Screening for lacI insert

Aim

The aim of this part of this experiment is to screen for the lacI insert.

Protocol

    1. Miniprep - the miniprep protocol was followed.
    2. Restriction digests - the restriction digests protocol was followed to allow the length of the DNA to be checked using single and double digest.
      • Single digest with Pst1
      • double digests with Pst1 and Xba1
    3. Gel electrophoresis - the gel electrophoresis protocol was followed.

Inference

  • Set up liquid broth culture in LB
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