"

From 2010.igem.org

Revision as of 10:50, 19 July 2010 (view source) Yessa (Talk | contribs) (→Summary) ← Older edit		Revision as of 13:56, 21 July 2010 (view source) Yessa (Talk | contribs) (→Summary) Newer edit →
Line 1:		Line 1:
	==Summary==		==Summary==
-	Screening for lacI insert:	+	*Screening for lacI insert:
-	#Miniprep	+	*#Miniprep
-	#Restriction digests	+	*#Restriction digests
-	#*Single digest with Pst1	+	*#*Single digest with Pst1
-	#*double digests with Pst1 and Xba1	+	*#*double digests with Pst1 and Xba1
-	#Gel electrophoresis	+	*#Gel electrophoresis
-	Set up liquid broth culture in LB	+	*Set up liquid broth culture in LB

---

Revision as of 13:56, 21 July 2010

Summary

• Screening for lacI insert:
  1. Miniprep
  2. Restriction digests
     • Single digest with Pst1
     • double digests with Pst1 and Xba1
  3. Gel electrophoresis

• Set up liquid broth culture in LB