http://2010.igem.org/wiki/index.php?title=Team:Newcastle/13_July_2010&feed=atom&action=historyTeam:Newcastle/13 July 2010 - Revision history2024-03-29T00:20:40ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Newcastle/13_July_2010&diff=148241&oldid=prevShethharsh08 at 19:38, 25 October 20102010-10-25T19:38:51Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=<del class="diffchange diffchange-inline">LacI BioBrick Construction</del>=</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=<ins class="diffchange diffchange-inline">Research</ins>=</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">==Aims==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">We still continued to research on the urease pathway.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*To use PCR to extract </del>''<del class="diffchange diffchange-inline">lacI</del>'' (<del class="diffchange diffchange-inline">promoter, ribosome</del>-<del class="diffchange diffchange-inline">binding site </del>(<del class="diffchange diffchange-inline">RBS</del>) <del class="diffchange diffchange-inline">& coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front </del>of <del class="diffchange diffchange-inline">red fluorescent protein </del>(<del class="diffchange diffchange-inline">RFP</del>). </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#Heidrich N, Chinali A, Gerth U and Brantl S. (2006). "The small untranslated RNA SR1 from the Bacillus subtilis genome is involved in the regulation of arginine catabolism". </ins>''<ins class="diffchange diffchange-inline">Molecular microbiology</ins>''<ins class="diffchange diffchange-inline">. 62</ins>(<ins class="diffchange diffchange-inline">2). 520</ins>-<ins class="diffchange diffchange-inline">36. </ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#Heidrich N, Moll I and Brantl S. </ins>(<ins class="diffchange diffchange-inline">2007</ins>)<ins class="diffchange diffchange-inline">. "In vitro analysis </ins>of <ins class="diffchange diffchange-inline">the interaction between the small RNA SR1 and its primary target ahrC mRNA". ''Nucleic acids research''. 35</ins>(<ins class="diffchange diffchange-inline">13</ins>). <ins class="diffchange diffchange-inline">4331-46. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==Materials==</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*The seven [[Team:Newcastle/12_July_2010|overnight cultures]]</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*Restriction enzyme ''Eco''R1</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==Protocol==</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*''E. coli'' DH5α was [[Team:Newcastle/Transformation_of_E._coli|transformed]] again with our ligation mix.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*The seven overnight cultures underwent [[Team:Newcastle/Qiagen Minipreps|plasmid extraction]].</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*The seven plasmid extracts were then [https://2010.igem.org/TeamNewcastleRestriction_digests| restriction digested] with ''Eco''R1 to linearise.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*The seven digests were then run on a gel.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==Results==</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Image:Newcastle LacI Gel 3.PNG|400px|centre]]</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">'''Figure 1''': Gel electrophoresis of the seven plasmid extracts which have been digested with ''Eco''R1.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*'''Lane 1''': 1 Kbp Molecular weight marker</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*'''Lane 2-8''': Colonies 1-7 in order</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*'''Lane 9''': 1 Kbp Molecular weight marker</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==Discussion==</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">This procedure was followed in order to determine whether our ''lacI'' had been ligated into the pSB1AT3 in the way we desired (i.e. in front of RFP). </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">The gel shows bands for colonies 1 and 2, that indicates that they contain vector only (size 3-3.5 Kbp) and for 3-7 that indicate that they contain vector plus RFP (size 4.5 Kbp), as with the original plasmid stock.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==Conclusion==</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">The conclusion that can be drawn from this gel is that the lacI has not been inserted into the plasmid as we desired. However due to the feint second band we are unsure as to whether plasmid has fully digested, to that end we will leave the digest running overnight and run the samples on a gel tomorrow. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Newcastle/footer}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Newcastle/footer}}</div></td></tr>
</table>Shethharsh08http://2010.igem.org/wiki/index.php?title=Team:Newcastle/13_July_2010&diff=145103&oldid=prevShethharsh08 at 14:40, 25 October 20102010-10-25T14:40:15Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">'''13 July 2010'''</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=LacI BioBrick Construction=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=LacI BioBrick Construction=</div></td></tr>
</table>Shethharsh08http://2010.igem.org/wiki/index.php?title=Team:Newcastle/13_July_2010&diff=47740&oldid=prevDeenatsu at 13:40, 11 August 20102010-08-11T13:40:21Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">'''13 July 2010'''</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=LacI BioBrick Construction=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=LacI BioBrick Construction=</div></td></tr>
</table>Deenatsuhttp://2010.igem.org/wiki/index.php?title=Team:Newcastle/13_July_2010&diff=47478&oldid=prevShethharsh08 at 08:17, 11 August 20102010-08-11T08:17:42Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Lane 9''': 1 Kbp Molecular weight marker</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Lane 9''': 1 Kbp Molecular weight marker</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">==Discussion==</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This procedure was followed in order to determine whether our ''lacI'' had been ligated into the pSB1AT3 in the way we desired (i.e. in front of RFP). </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This procedure was followed in order to determine whether our ''lacI'' had been ligated into the pSB1AT3 in the way we desired (i.e. in front of RFP). </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The gel shows bands for colonies 1 and 2 that <del class="diffchange diffchange-inline">indicate </del>that they contain vector only (size 3-3.5 Kbp) and for 3-7 that indicate that they contain vector plus RFP (size 4.5 Kbp), as with the original plasmid stock.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The gel shows bands for colonies 1 and 2<ins class="diffchange diffchange-inline">, </ins>that <ins class="diffchange diffchange-inline">indicates </ins>that they contain vector only (size 3-3.5 Kbp) and for 3-7 that indicate that they contain vector plus RFP (size 4.5 Kbp), as with the original plasmid stock.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Conclusion==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Conclusion==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The conclusion that can be drawn from this gel is that the lacI has not been inserted into the plasmid as we desired. However due to the feint second band we are unsure as to whether plasmid has fully digested, to that end we will leave the digest running overnight and run the samples on a gel tomorrow. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The conclusion that can be drawn from this gel is that the lacI has not been inserted into the plasmid as we desired. However due to the feint second band we are unsure as to whether plasmid has fully digested, to that end we will leave the digest running overnight and run the samples on a gel tomorrow. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Newcastle/footer}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Newcastle/footer}}</div></td></tr>
</table>Shethharsh08http://2010.igem.org/wiki/index.php?title=Team:Newcastle/13_July_2010&diff=46834&oldid=prevPhilipHall: /* Results */2010-08-10T15:29:08Z<p><span class="autocomment">Results</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This procedure was followed in order to determine whether our ''lacI'' had been ligated into the pSB1AT3 in the way we desired (i.e. in front of RFP). </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This procedure was followed in order to determine whether our ''lacI'' had been ligated into the pSB1AT3 in the way we desired (i.e. in front of RFP). </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The gel shows bands for colonies 1 and 2 that indicate that they contain vector only (size 3 Kbp) and for 3-7 that indicate that they contain vector plus RFP (size 4.5 Kbp), as with the original plasmid stock.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The gel shows bands for colonies 1 and 2 that indicate that they contain vector only (size 3<ins class="diffchange diffchange-inline">-3.5 </ins>Kbp) and for 3-7 that indicate that they contain vector plus RFP (size 4.5 Kbp), as with the original plasmid stock.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Conclusion==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Conclusion==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The conclusion that can be drawn from this gel is that the lacI has not been inserted into the plasmid as we desired. However due to the feint second band we are unsure as to whether plasmid has fully digested, to that end we will leave the digest running overnight and run the samples on a gel tomorrow. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The conclusion that can be drawn from this gel is that the lacI has not been inserted into the plasmid as we desired. However due to the feint second band we are unsure as to whether plasmid has fully digested, to that end we will leave the digest running overnight and run the samples on a gel tomorrow. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Newcastle/footer}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Newcastle/footer}}</div></td></tr>
</table>PhilipHallhttp://2010.igem.org/wiki/index.php?title=Team:Newcastle/13_July_2010&diff=46581&oldid=prevPhilipHall: /* Conclusion */2010-08-10T11:02:36Z<p><span class="autocomment">Conclusion</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Conclusion==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Conclusion==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The conclusion that can be drawn from this gel is that the lacI has not been inserted into the plasmid as we desired. However due to <del class="diffchange diffchange-inline">some confusion </del>as to the <del class="diffchange diffchange-inline">actual size of </del>the <del class="diffchange diffchange-inline">vector </del>a <del class="diffchange diffchange-inline">set of double digests will be performed </del>tomorrow <del class="diffchange diffchange-inline">to try to extract insert form the plasmid if any is present</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The conclusion that can be drawn from this gel is that the lacI has not been inserted into the plasmid as we desired. However due to <ins class="diffchange diffchange-inline">the feint second band we are unsure </ins>as to <ins class="diffchange diffchange-inline">whether plasmid has fully digested, to that end we will leave </ins>the <ins class="diffchange diffchange-inline">digest running overnight and run </ins>the <ins class="diffchange diffchange-inline">samples on </ins>a <ins class="diffchange diffchange-inline">gel </ins>tomorrow. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Newcastle/footer}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:Newcastle/footer}}</div></td></tr>
</table>PhilipHallhttp://2010.igem.org/wiki/index.php?title=Team:Newcastle/13_July_2010&diff=46565&oldid=prevPhilipHall: /* Materials */2010-08-10T10:28:28Z<p><span class="autocomment">Materials</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Materials==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Materials==</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*<del class="diffchange diffchange-inline">Seven </del>[[Team:Newcastle/12_July_2010|overnight cultures]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*<ins class="diffchange diffchange-inline">The seven </ins>[[Team:Newcastle/12_July_2010|overnight cultures]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Restriction enzyme ''Eco''R1</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Restriction enzyme ''Eco''R1</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Protocol==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Protocol==</div></td></tr>
</table>PhilipHallhttp://2010.igem.org/wiki/index.php?title=Team:Newcastle/13_July_2010&diff=46563&oldid=prevPhilipHall: /* Results */2010-08-10T10:27:32Z<p><span class="autocomment">Results</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>This procedure was followed in order to determine whether our ''lacI'' had been ligated into the pSB1AT3. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>This procedure was followed in order to determine whether our ''lacI'' had been ligated into the pSB1AT3 <ins class="diffchange diffchange-inline">in the way we desired (i.e. in front of RFP)</ins>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The gel shows bands for colonies 1 and 2 that indicate that they contain vector only (size 3 Kbp) and for 3-7 that indicate that they contain vector plus RFP (size 4.5 Kbp), as with the original plasmid stock.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The gel shows bands for colonies 1 and 2 that indicate that they contain vector only (size 3 Kbp) and for 3-7 that indicate that they contain vector plus RFP (size 4.5 Kbp), as with the original plasmid stock.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>PhilipHallhttp://2010.igem.org/wiki/index.php?title=Team:Newcastle/13_July_2010&diff=46562&oldid=prevPhilipHall: /* Results */2010-08-10T10:26:47Z<p><span class="autocomment">Results</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>This procedure was followed in order to determine whether our ''lacI'' had been ligated into the <del class="diffchange diffchange-inline">plasmid vector</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>This procedure was followed in order to determine whether our ''lacI'' had been ligated into the <ins class="diffchange diffchange-inline">pSB1AT3</ins>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The gel shows bands for colonies 1 and 2 that indicate that they contain vector only (size 3 Kbp) and for 3-7 that indicate that they contain vector plus RFP (size 4.5 Kbp), as with the original plasmid stock.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The gel shows bands for colonies 1 and 2 that indicate that they contain vector only (size 3 Kbp) and for 3-7 that indicate that they contain vector plus RFP (size 4.5 Kbp), as with the original plasmid stock.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>PhilipHallhttp://2010.igem.org/wiki/index.php?title=Team:Newcastle/13_July_2010&diff=46560&oldid=prevPhilipHall: /* Results */2010-08-10T10:26:11Z<p><span class="autocomment">Results</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Lane 2-8''': Colonies 1-7 in order</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Lane 2-8''': Colonies 1-7 in order</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Lane 9''': 1 Kbp Molecular weight marker</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Lane 9''': 1 Kbp Molecular weight marker</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*</del>This procedure was followed in order to determine whether our ''lacI'' had been ligated into the plasmid vector. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>This procedure was followed in order to determine whether our ''lacI'' had been ligated into the plasmid vector. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The gel shows bands for colonies 1 and 2 that indicate that they contain vector only (size 3 Kbp) and for 3-7 that indicate that they contain vector plus RFP (size 4.5 Kbp), as with the original plasmid stock.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The gel shows bands for colonies 1 and 2 that indicate that they contain vector only (size 3 Kbp) and for 3-7 that indicate that they contain vector plus RFP (size 4.5 Kbp), as with the original plasmid stock.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>PhilipHall