Team:Newcastle/13 July 2010
From 2010.igem.org
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==Miniprep== | ==Miniprep== | ||
*For this step the [https://2010.igem.org/Team:Newcastle/Qiagen_Minipreps| Qiagen Minipreps] protocol was followed. The 7 colonies that grew on the plates from yesterday were cultured ovenight on a shaking shelf at 37°C in universal tubes. | *For this step the [https://2010.igem.org/Team:Newcastle/Qiagen_Minipreps| Qiagen Minipreps] protocol was followed. The 7 colonies that grew on the plates from yesterday were cultured ovenight on a shaking shelf at 37°C in universal tubes. | ||
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+ | ==Restriction digests== | ||
+ | *The [https://2010.igem.org/TeamNewcastleRestriction_digests| restriction digest] protocol was followed for this step. | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} |
Revision as of 15:50, 3 August 2010
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Transformation
- E. coli DH5alpha was transformed again with our plasmid. The transformation protocol was followed.
Miniprep
- For this step the Qiagen Minipreps protocol was followed. The 7 colonies that grew on the plates from yesterday were cultured ovenight on a shaking shelf at 37°C in universal tubes.
Restriction digests
- The restriction digest protocol was followed for this step.