Team:Newcastle/13 July 2010
From 2010.igem.org
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==Miniprep== | ==Miniprep== | ||
- | *For this step the [https://2010.igem.org/Team:Newcastle/Qiagen_Minipreps| Qiagen Minipreps protocol] was followed. | + | *For this step the [https://2010.igem.org/Team:Newcastle/Qiagen_Minipreps| Qiagen Minipreps protocol] was followed. The 7 colonies that grew on the plates from yesterday were cultured ovenight on a shaking shelf at 37°C in universal tubes. |
- | The 7 colonies that grew on the plates from yesterday were cultured ovenight on a shaking shelf at 37°C in universal tubes. | + | |
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} |
Revision as of 15:41, 3 August 2010
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Transformation
- E. coli DH5alpha was transformed again with our plasmid. The transformation protocol was followed.
Miniprep
- For this step the Qiagen Minipreps protocol was followed. The 7 colonies that grew on the plates from yesterday were cultured ovenight on a shaking shelf at 37°C in universal tubes.