Team:Newcastle/13 July 2010
From 2010.igem.org
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{{Team:Newcastle/mainbanner}} | {{Team:Newcastle/mainbanner}} | ||
- | Transformation | + | ==Transformation== |
- | * ''E. coli'' DH5alpha was transformed again with our plasmid. The [https://2010.igem.org/TeamNewcastleTransformation_of_E._coli#Protocol| transformation protocol] was followed. | + | *''E. coli'' DH5alpha was transformed again with our plasmid. The [https://2010.igem.org/TeamNewcastleTransformation_of_E._coli#Protocol| transformation protocol] was followed. |
- | Miniprep | + | ==Miniprep== |
- | For this step the [https://2010.igem.org/Team:Newcastle/Qiagen_Minipreps| Qiagen Minipreps protocol] was followed. | + | *For this step the [https://2010.igem.org/Team:Newcastle/Qiagen_Minipreps| Qiagen Minipreps protocol] was followed. |
The 7 colonies that grew on the plates from yesterday were cultured ovenight on a shaking shelf at 37°C in universal tubes. | The 7 colonies that grew on the plates from yesterday were cultured ovenight on a shaking shelf at 37°C in universal tubes. | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} |
Revision as of 15:39, 3 August 2010
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Transformation
- E. coli DH5alpha was transformed again with our plasmid. The transformation protocol was followed.
Miniprep
- For this step the Qiagen Minipreps protocol was followed.
The 7 colonies that grew on the plates from yesterday were cultured ovenight on a shaking shelf at 37°C in universal tubes.