Team:Newcastle/13 July 2010

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Summary:
 
Transformation  
Transformation  
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* transforming ''E. coli'' DH5alpha with our plasmid. The [https://2010.igem.org/TeamNewcastleTransformation_of_E._coli#Protocol| transformation protocol] was used.  
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* ''E. coli'' DH5alpha was transformed again with our plasmid. The [https://2010.igem.org/TeamNewcastleTransformation_of_E._coli#Protocol| transformation protocol] was followed.  
Miniprep  
Miniprep  

Revision as of 15:31, 3 August 2010

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Transformation

Miniprep The 7 colonies that grew on the plates from yesterday were cultured ovenight on a shaking shelf at 37°C in universal tubes.

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