Latest revision as of 19:38, 25 October 2010

Research

We still continued to research on the urease pathway.

Heidrich N, Chinali A, Gerth U and Brantl S. (2006). "The small untranslated RNA SR1 from the Bacillus subtilis genome is involved in the regulation of arginine catabolism". Molecular microbiology. 62(2). 520-36.
Heidrich N, Moll I and Brantl S. (2007). "In vitro analysis of the interaction between the small RNA SR1 and its primary target ahrC mRNA". Nucleic acids research. 35(13). 4331-46.

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-=LacI BioBrick Construction=
+=Research=
-==Aims==
+We still continued to research on the urease pathway.
-To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
+#Heidrich N, Chinali A, Gerth U and Brantl S. (2006). "The small untranslated RNA SR1 from the Bacillus subtilis genome is involved in the regulation of arginine catabolism". ''Molecular microbiology''. 62(2). 520-36.
+#Heidrich N, Moll I and Brantl S. (2007). "In vitro analysis of the interaction between the small RNA SR1 and its primary target ahrC mRNA". ''Nucleic acids research''. 35(13). 4331-46.
-==Materials==
-Seven [[Team:Newcastle/12_July_2010|overnight cultures]]
-Restriction enzyme ecoR1
-==Protocol==
-*''E. coli'' DH5alpha was [https://2010.igem.org/TeamNewcastleTransformation_of_E._coli#Protocol|transformed]] again with our ligation mix.
-*The seven overnight cultures underwent [[Team:Newcastle/Qiagen Minipreps|plasmid extraction]].
-*The seven plasmid extracts were then [https://2010.igem.org/TeamNewcastleRestriction_digests| restriction digested] with ecoR1 to linearise.
-*The seven digests were then run on a gel.
-==Results==
-This procedure was followed in order to determine whether our ''lacI'' had been ligated into the plasmid vector. The Gel shows that
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