Latest revision as of 19:38, 25 October 2010

Research

We still continued to research on the urease pathway.

Heidrich N, Chinali A, Gerth U and Brantl S. (2006). "The small untranslated RNA SR1 from the Bacillus subtilis genome is involved in the regulation of arginine catabolism". Molecular microbiology. 62(2). 520-36.
Heidrich N, Moll I and Brantl S. (2007). "In vitro analysis of the interaction between the small RNA SR1 and its primary target ahrC mRNA". Nucleic acids research. 35(13). 4331-46.

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-==LacI BioBrick Construction==
+=Research=
-===Aims===
+We still continued to research on the urease pathway.
-To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
+#Heidrich N, Chinali A, Gerth U and Brantl S. (2006). "The small untranslated RNA SR1 from the Bacillus subtilis genome is involved in the regulation of arginine catabolism". ''Molecular microbiology''. 62(2). 520-36.
+#Heidrich N, Moll I and Brantl S. (2007). "In vitro analysis of the interaction between the small RNA SR1 and its primary target ahrC mRNA". ''Nucleic acids research''. 35(13). 4331-46.
-===Transformation===
-*''E. coli'' DH5alpha was transformed again with our plasmid. The [https://2010.igem.org/TeamNewcastleTransformation_of_E._coli#Protocol| transformation protocol] was followed.
-===Miniprep===
-*For this step the [https://2010.igem.org/Team:Newcastle/Qiagen_Minipreps| Qiagen Minipreps] protocol was followed. The 7 colonies that grew on the plates from yesterday were cultured ovenight on a shaking shelf at 37°C in universal tubes.
-===Restriction digests===
-*The [https://2010.igem.org/TeamNewcastleRestriction_digests| restriction digest] protocol was followed for this step.
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