Latest revision as of 19:38, 25 October 2010

Research

We still continued to research on the urease pathway.

Heidrich N, Chinali A, Gerth U and Brantl S. (2006). "The small untranslated RNA SR1 from the Bacillus subtilis genome is involved in the regulation of arginine catabolism". Molecular microbiology. 62(2). 520-36.
Heidrich N, Moll I and Brantl S. (2007). "In vitro analysis of the interaction between the small RNA SR1 and its primary target ahrC mRNA". Nucleic acids research. 35(13). 4331-46.

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-==Transformation==
+=Research=
-*''E. coli'' DH5alpha was transformed again with our plasmid. The [https://2010.igem.org/TeamNewcastleTransformation_of_E._coli#Protocol| transformation protocol] was followed.
-==Miniprep==
+We still continued to research on the urease pathway.
-*For this step the [https://2010.igem.org/Team:Newcastle/Qiagen_Minipreps| Qiagen Minipreps protocol] was followed. The 7 colonies that grew on the plates from yesterday were cultured ovenight on a shaking shelf at 37°C in universal tubes.
+#Heidrich N, Chinali A, Gerth U and Brantl S. (2006). "The small untranslated RNA SR1 from the Bacillus subtilis genome is involved in the regulation of arginine catabolism". ''Molecular microbiology''. 62(2). 520-36.
+#Heidrich N, Moll I and Brantl S. (2007). "In vitro analysis of the interaction between the small RNA SR1 and its primary target ahrC mRNA". ''Nucleic acids research''. 35(13). 4331-46.
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