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Team:Newcastle/10 August 2010
From 2010.igem.org
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Contents |
Gel extraction of rocF BioBrick components
Gel Electrophoresis for Amplified Pspac_oid promoter, Double terminator and Plasmid Vector pSB1C3
Aim
The aim of the experiment is to perform gel electrophoresis for the amplified fragments of Pspac_oid promoter, double terminator and plasmid vector pSB1C3 and thus perform gel exraction of the required bands.
Materials and Protocol
Please refer to: Gel electrophoresis.
Result
The experiment is divided in two separate gels. For plasmid vector pSB1C3, we used 1% agarose gel but for Psapc_oid and double terminator fragment we used 1.5% agarose gel for a better resolution. We used 1Kb DNA ladder (Promega) for the gel containing plasmid vector while we used 100bp DNA ladder (Promega) for the gel containing Pspac_oid and double terminator fragment.
| Pspac_oid pormoter | |
|---|---|
| Size of the Fragment (in bp) | 148 approx. |
Table 3: Table represents the size of the Pspac_oid fragment represented as bands on the gel in all the lanes.
Discussion
We found two bands in the all lanes out of which one is of approximately of 150 bp is size and the other band is of 80 bp approximately in size.
Conclusion
Gibson assembly of rocF BioBrick
   
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