Gel extraction of rocF BioBrick components

Gel Electrophoresis for Amplified Pspac_oid promoter, Double terminator and Plasmid Vector pSB1C3

Aim

The aim of the experiment is to perform gel electrophoresis for the amplified fragments of Pspac_oid promoter, double terminator and plasmid vector pSB1C3 and thus perform gel exraction of the required bands.

Materials and Protocol

Please refer to: Gel electrophoresis.

Result

The experiment is divided in two separate gels. For plasmid vector pSB1C3, we used 1% agarose gel but for Psapc_oid and double terminator fragment we used 1.5% agarose gel for a better resolution.

• Lane 1: 1kb DNA ladder
• Lane 2: Plamid pMutin4 containing Pspac_oid promoter
• Lane 3: Plamid pMutin4 containing Pspac_oid promoter
• Lane 4: Plamid pMK-RQ (kinA BioBrick) containing Pspac_oid promoter
• Lane 5: Plamid pMK-RQ (kinA BioBrick) containing Pspac_oid promoter
• Lane 6: Plamid pMK-RO (stchastic switch BioBrick) containing Pspac_oid promoter
• Lane 7: Plamid pMK-RO (stchastic switch BioBrick) containing Pspac_oid promoter
• Lane 8: 100bp DNA ladder

Table 3: Table represents the size of the Pspac_oid fragment represented as bands on the gel in all the lanes.

Discussion

We found two bands in the all lanes out of which one is of approximately of 150 bp is size and the other band is of 80 bp approximately in size.

Conclusion

Gibson assembly of rocF BioBrick