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Team:Newcastle/10 August 2010
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{{Team:Newcastle/mainbanner}} | {{Team:Newcastle/mainbanner}} | ||
| - | + | =Gel extraction of ''rocF'' BioBrick components= | |
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| + | ==Gel Electrophoresis for Amplified Pspac_oid promoter== | ||
| + | |||
| + | ===Aim=== | ||
| + | The aim of the experiment is to perform gel electrophoresis for the two PCR reactions Pspac_oid promoter amplified from plasmid pMutin4 and from plasmid pMK-RQ containing Biobrick ''kinA'' and plasmid pMK-RQ containing stochastic switch developed by Team Newcastle 2009 which took place today morning and thus confirm that they were successful. | ||
| + | |||
| + | ===Materials and Protocol=== | ||
| + | Please refer to: [[Team:Newcastle/Gel electrophoresis| Gel electrophoresis]]. | ||
| + | |||
| + | ===Result=== | ||
| + | |||
| + | |||
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| + | '''Figure 1''': Gel electrophoresis of the ''lacI'' and Pspac_oid promoter. | ||
| + | |||
| + | * '''Lane 1''': 1kb DNA ladder | ||
| + | * '''Lane 2''': Plamid pMutin4 containing Pspac_oid promoter | ||
| + | * '''Lane 3''': Plamid pMutin4 containing Pspac_oid promoter | ||
| + | * '''Lane 4''': Plamid pMK-RQ (''kinA'' BioBrick) containing Pspac_oid promoter | ||
| + | * '''Lane 5''': Plamid pMK-RQ (''kinA'' BioBrick) containing Pspac_oid promoter | ||
| + | * '''Lane 6''': Plamid pMK-RO (stchastic switch BioBrick) containing Pspac_oid promoter | ||
| + | * '''Lane 7''': Plamid pMK-RO (stchastic switch BioBrick) containing Pspac_oid promoter | ||
| + | * '''Lane 8''': 100bp DNA ladder | ||
| + | |||
| + | {|border=1 | ||
| + | |- | ||
| + | ! | ||
| + | !'''Pspac_oid pormoter''' | ||
| + | |- | ||
| + | |'''Size of the Fragment (in bp)''' | ||
| + | |148 approx. | ||
| + | |} | ||
| + | '''Table 3''': Table represents the size of the Pspac_oid fragment represented as bands on the gel in all the lanes. | ||
| + | |||
| + | ===Discussion=== | ||
| + | We found two bands in the all lanes out of which one is of approximately of 150 bp is size and the other band is of 80 bp approximately in size. | ||
| + | |||
| + | ===Conclusion=== | ||
==Gibson assembly of ''rocF'' BioBrick== | ==Gibson assembly of ''rocF'' BioBrick== | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Revision as of 11:02, 10 August 2010
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Contents |
Gel extraction of rocF BioBrick components
Gel Electrophoresis for Amplified Pspac_oid promoter
Aim
The aim of the experiment is to perform gel electrophoresis for the two PCR reactions Pspac_oid promoter amplified from plasmid pMutin4 and from plasmid pMK-RQ containing Biobrick kinA and plasmid pMK-RQ containing stochastic switch developed by Team Newcastle 2009 which took place today morning and thus confirm that they were successful.
Materials and Protocol
Please refer to: Gel electrophoresis.
Result
Figure 1: Gel electrophoresis of the lacI and Pspac_oid promoter.
- Lane 1: 1kb DNA ladder
- Lane 2: Plamid pMutin4 containing Pspac_oid promoter
- Lane 3: Plamid pMutin4 containing Pspac_oid promoter
- Lane 4: Plamid pMK-RQ (kinA BioBrick) containing Pspac_oid promoter
- Lane 5: Plamid pMK-RQ (kinA BioBrick) containing Pspac_oid promoter
- Lane 6: Plamid pMK-RO (stchastic switch BioBrick) containing Pspac_oid promoter
- Lane 7: Plamid pMK-RO (stchastic switch BioBrick) containing Pspac_oid promoter
- Lane 8: 100bp DNA ladder
| Pspac_oid pormoter | |
|---|---|
| Size of the Fragment (in bp) | 148 approx. |
Table 3: Table represents the size of the Pspac_oid fragment represented as bands on the gel in all the lanes.
Discussion
We found two bands in the all lanes out of which one is of approximately of 150 bp is size and the other band is of 80 bp approximately in size.
Conclusion
Gibson assembly of rocF BioBrick
   
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