http://2010.igem.org/wiki/index.php?title=Team:Newcastle&feed=atom&action=history
Team:Newcastle - Revision history
2024-03-29T05:47:50Z
Revision history for this page on the wiki
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http://2010.igem.org/wiki/index.php?title=Team:Newcastle&diff=209064&oldid=prev
Yessa at 19:13, 25 November 2010
2010-11-25T19:13:47Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our project is summarised in the diagram below:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our project is summarised in the diagram below:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:newcastle_summary.png|<ins class="diffchange diffchange-inline">600px</ins>|centre]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:newcastle_summary.png|<del class="diffchange diffchange-inline">900px</del>|centre]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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Yessa
http://2010.igem.org/wiki/index.php?title=Team:Newcastle&diff=209063&oldid=prev
Yessa at 19:11, 25 November 2010
2010-11-25T19:11:09Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<span style="color:Goldenrod">'''GOLD:'''</span></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<span style="color:Goldenrod">'''GOLD:'''</span></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|In order to obtain a gold, we investigated the benefits of an e-Science approach, focusing on workflows, to synthetic biology. Details can be found at [[Team:Newcastle/E-Science|here]]. This part of the project resulted in us proposing a new standard for a RESTful API which facilitates the discovery and publication of models of functional biological units. The standard has been submitted to the BioBricks Foundation as [[BBFRFC66|BBF RFC 66]<del class="diffchange diffchange-inline">]. We have also improved on existing BioBrick parts to produce hyperspankoid: [http://partsregistry.org/Part:BBa_K302003 BBa_K302003</del>].</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|In order to obtain a gold, we investigated the benefits of an e-Science approach, focusing on workflows, to synthetic biology. Details can be found at [[Team:Newcastle/E-Science|here]]. This part of the project resulted in us proposing a new standard for a RESTful API which facilitates the discovery and publication of models of functional biological units. The standard has been submitted to the BioBricks Foundation as [[BBFRFC66|BBF RFC 66]].</div></td></tr>
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Yessa
http://2010.igem.org/wiki/index.php?title=Team:Newcastle&diff=204884&oldid=prev
Yessa at 02:08, 28 October 2010
2010-10-28T02:08:54Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Summary of Our Achievements===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Summary of Our Achievements===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{|style cellpadding="<del class="diffchange diffchange-inline">20</del>" cellspacing="0"</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{|style cellpadding="<ins class="diffchange diffchange-inline">14</ins>" cellspacing="0"</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<span style="color:Sienna">'''BRONZE:'''</span></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<span style="color:Sienna">'''BRONZE:'''</span></div></td></tr>
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Yessa
http://2010.igem.org/wiki/index.php?title=Team:Newcastle&diff=204627&oldid=prev
Yessa at 02:01, 28 October 2010
2010-10-28T02:01:36Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Newcastle_iGEM_Teampic.jpeg|centre|468px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Newcastle_iGEM_Teampic.jpeg|centre|468px]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Project Description===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Project Description===</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BacillaFilla, an engineered ''Bacillus subtilis'', aims to repair [[Team:Newcastle/problem|cracks in concrete]] which can cause catastrophic structural failure. BacillaFilla would be applied to structures by spraying onto their surfaces.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BacillaFilla, an engineered ''Bacillus subtilis'', aims to repair [[Team:Newcastle/problem|cracks in concrete]] which can cause catastrophic structural failure. BacillaFilla would be applied to structures by spraying onto their surfaces.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>BacillaFilla would swim deep into the cracks. Repair would be effected by production of [[Team:Newcastle/Urease|CaCO<sub>3</sub>]], [[Team:Newcastle/Filamentous_Cells|filamentous ''<del class="diffchange diffchange-inline">Bacillus </del>subtilis'' cells]] and [[Team:Newcastle/glue|levansucrose glue]]. CaCO<sub>3</sub> expands at the same rate as concrete, making it an ideal filler. Filamentous ''<del class="diffchange diffchange-inline">Bacillus </del>subtilis'' cells have similar tensile strength to the synthetic fibres used in fibre-reinforced concrete, and provide reinforcement. Levansucrose glues CaCO<sub>3</sub> and filamentous cells in place.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>BacillaFilla would swim deep into the cracks. Repair would be effected by production of [[Team:Newcastle/Urease|CaCO<sub>3</sub>]], [[Team:Newcastle/Filamentous_Cells|filamentous ''<ins class="diffchange diffchange-inline">B. </ins>subtilis'' cells]] and [[Team:Newcastle/glue|levansucrose glue]]. CaCO<sub>3</sub> expands at the same rate as concrete, making it an ideal filler. Filamentous ''<ins class="diffchange diffchange-inline">B. </ins>subtilis'' cells have similar tensile strength to the synthetic fibres used in fibre-reinforced concrete, and provide reinforcement. Levansucrose glues CaCO<sub>3</sub> and filamentous cells in place.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''B. subtilis'' 168 sporulates, making it ideal for storage and transportation. The cells can be [[Team:Newcastle/solution#Alkalinity_resistance|made to be tolerant to concrete's high pH]].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''B. subtilis'' 168 sporulates, making it ideal for storage and transportation. The cells can be [[Team:Newcastle/solution#Alkalinity_resistance|made to be tolerant to concrete's high pH]].</div></td></tr>
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Yessa
http://2010.igem.org/wiki/index.php?title=Team:Newcastle&diff=204591&oldid=prev
Yessa at 01:59, 28 October 2010
2010-10-28T01:59:38Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our project is summarised in the diagram below:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our project is summarised in the diagram below:</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:newcastle_summary.png|900px|centre]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:newcastle_summary.png|900px|centre]]</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">===Summary of Our Achievements===</ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">! colspan="2" |<font size=4> <center>'''Summary of achievements:'''</center></font></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<span style="color:Sienna">'''BRONZE:'''</span></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|<span style="color:Sienna">'''BRONZE:'''</span></div></td></tr>
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Yessa
http://2010.igem.org/wiki/index.php?title=Team:Newcastle&diff=204028&oldid=prev
Yessa: /* Project Description */
2010-10-28T01:23:03Z
<p><span class="autocomment">Project Description</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We designed a [[Team:Newcastle/Swarming|swarming]] BioBrick part for repairing ''B. subtilis'' 168's defective ''swrA'' and ''sfp'' genes, regaining motility. At the end of the crack the quorum sensing peptide [[Team:Newcastle/End_of_crack_%26_signalling_system|subtilin]] triggers a co-ordinated population response from a [[Team:Newcastle/End_of_crack_%26_signalling_system#2008Brick|subtilin-inducible promoter]]. Upregulating ''SR1'' and ''rocF'' promotes arginine and urea production, increasing exogenous CaCO<sub>3</sub> deposition. Over-producing YneA induces the filamentous cell phenotype, while SacB converts extracellular sucrose to levan glue.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We designed a [[Team:Newcastle/Swarming|swarming]] BioBrick part for repairing ''B. subtilis'' 168's defective ''swrA'' and ''sfp'' genes, regaining motility. At the end of the crack the quorum sensing peptide [[Team:Newcastle/End_of_crack_%26_signalling_system|subtilin]] triggers a co-ordinated population response from a [[Team:Newcastle/End_of_crack_%26_signalling_system#2008Brick|subtilin-inducible promoter]]. Upregulating ''SR1'' and ''rocF'' promotes arginine and urea production, increasing exogenous CaCO<sub>3</sub> deposition. Over-producing YneA induces the filamentous cell phenotype, while SacB converts extracellular sucrose to levan glue.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>To protect the environment our project also includes a design for a [[Team:Newcastle/Non-target-environment_kill_switch| kill switch]].</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>To protect the environment our project also includes a design for a [[Team:Newcastle/Non-target-environment_kill_switch| kill switch]]. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Our project is summarised in the diagram below:</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:newcastle_summary.png|900px|centre]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:newcastle_summary.png|900px|centre]]</div></td></tr>
</table>
Yessa
http://2010.igem.org/wiki/index.php?title=Team:Newcastle&diff=203906&oldid=prev
Yessa at 01:19, 28 October 2010
2010-10-28T01:19:31Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__NOTOC__{{Team:Newcastle/mainbanner}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__NOTOC__{{Team:Newcastle/mainbanner}}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=BacillaFilla: <del class="diffchange diffchange-inline">fixing cracks </del>in <del class="diffchange diffchange-inline">concrete</del>= </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=BacillaFilla: <ins class="diffchange diffchange-inline">Fixing Cracks </ins>in <ins class="diffchange diffchange-inline">Concrete</ins>= </div></td></tr>
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Yessa
http://2010.igem.org/wiki/index.php?title=Team:Newcastle&diff=203787&oldid=prev
Yessa: /* BacillaFilla: Fixing Cracks in Concrete */
2010-10-28T01:16:47Z
<p><span class="autocomment">BacillaFilla: Fixing Cracks in Concrete</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=BacillaFilla: <del class="diffchange diffchange-inline">Fixing Cracks </del>in <del class="diffchange diffchange-inline">Concrete</del>= </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=BacillaFilla: <ins class="diffchange diffchange-inline">fixing cracks </ins>in <ins class="diffchange diffchange-inline">concrete</ins>= </div></td></tr>
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Yessa
http://2010.igem.org/wiki/index.php?title=Team:Newcastle&diff=201073&oldid=prev
Yessa at 23:45, 27 October 2010
2010-10-27T23:45:21Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:newcastle_summary.png|<del class="diffchange diffchange-inline">950px</del>|centre]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:newcastle_summary.png|<ins class="diffchange diffchange-inline">900px</ins>|centre]]</div></td></tr>
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Yessa
http://2010.igem.org/wiki/index.php?title=Team:Newcastle&diff=200605&oldid=prev
Yessa: /* BacillaFilla: Fixing Cracks in Concrete */
2010-10-27T23:24:03Z
<p><span class="autocomment">BacillaFilla: Fixing Cracks in Concrete</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:newcastle_summary.png|<del class="diffchange diffchange-inline">900px</del>|centre]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:newcastle_summary.png|<ins class="diffchange diffchange-inline">950px</ins>|centre]]</div></td></tr>
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Yessa