Team:Newcastle

From 2010.igem.org

(Difference between revisions)
(Project Description)
Line 8: Line 8:
<div style="text-align:justify;padding:5px">
<div style="text-align:justify;padding:5px">
===Project Description===
===Project Description===
-
BacillaFilla, an engineered Bacillus subtilis, aims to repair [[Team:Newcastle/problem|Microcracks in concrete]], which can cause catastrophic structural failure. BacillaFilla would be applied to structures by spraying onto their surface.
+
BacillaFilla, an engineered ''Bacillus subtilis'', aims to repair [[Team:Newcastle/problem|Microcracks in concrete]], which can cause catastrophic structural failure. BacillaFilla would be applied to structures by spraying onto their surface.
The Bacillus swims deep into the microcracks. Repair is effected by production of CaCO3, [[Team:Newcastle/Filamentous_Cells| Filamentous ''Bacillus subtilis'' cells]]  and [[Team:Newcastle/glue|Levansucrose ]]. [[Team:Newcastle/Urease| CaCO<sub>3</sub>]] expands at the same rate as concrete, making it the ideal filler. A filamentous cell mesh provides reinforcement. Levansucrose glues CaCO3 and filamentous cells in place.
The Bacillus swims deep into the microcracks. Repair is effected by production of CaCO3, [[Team:Newcastle/Filamentous_Cells| Filamentous ''Bacillus subtilis'' cells]]  and [[Team:Newcastle/glue|Levansucrose ]]. [[Team:Newcastle/Urease| CaCO<sub>3</sub>]] expands at the same rate as concrete, making it the ideal filler. A filamentous cell mesh provides reinforcement. Levansucrose glues CaCO3 and filamentous cells in place.
-
B. subtilis 168 sporulates, making it ideal for storage and transportation. The cells are naturally tolerant to concrete's high pH. We repaired 168's defective [[Team:Newcastle/Swarming|swrA and sfp ]], regaining motility. At the end of the crack the [[Team:Newcastle/End_of_crack_%26_signalling_system|quorum communication ]]peptide subtilin triggers a co-ordinated population response from a [[Team:Newcastle/End_of_crack_%26_signalling_system#2008Brick|subtilin-inducible promoter ]] . Upregulating SR1 and rocF promotes arginine and urea production, increasing exogenous  CaCO3 deposition. Over-producing  yneA induces  the filamentous cell phenotype, while SacB converts extracellular sucrose to levansucrose glue.
+
''B. subtilis'' 168 sporulates, making it ideal for storage and transportation. The cells are naturally tolerant to concrete's high pH. We repaired ''B. subtilis'' 168's defective [[Team:Newcastle/Swarming|swrA and sfp ]], regaining motility. At the end of the crack the [[Team:Newcastle/End_of_crack_%26_signalling_system|quorum communication ]]peptide subtilin triggers a co-ordinated population response from a [[Team:Newcastle/End_of_crack_%26_signalling_system#2008Brick|subtilin-inducible promoter ]] . Upregulating SR1 and rocF promotes arginine and urea production, increasing exogenous  CaCO3 deposition. Over-producing  yneA induces  the filamentous cell phenotype, while SacB converts extracellular sucrose to levansucrose glue.
To protect the environment our project will also include a design for a [[Team:Newcastle/Non-target-environment_kill_switch| kill switch]].
To protect the environment our project will also include a design for a [[Team:Newcastle/Non-target-environment_kill_switch| kill switch]].

Revision as of 12:17, 22 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

BacillaFilla: Fixing cracks in Concrete

Newcastle iGEM Teampic.jpeg


Project Description

BacillaFilla, an engineered Bacillus subtilis, aims to repair Microcracks in concrete, which can cause catastrophic structural failure. BacillaFilla would be applied to structures by spraying onto their surface. The Bacillus swims deep into the microcracks. Repair is effected by production of CaCO3, Filamentous Bacillus subtilis cells and Levansucrose . CaCO3 expands at the same rate as concrete, making it the ideal filler. A filamentous cell mesh provides reinforcement. Levansucrose glues CaCO3 and filamentous cells in place. B. subtilis 168 sporulates, making it ideal for storage and transportation. The cells are naturally tolerant to concrete's high pH. We repaired B. subtilis 168's defective swrA and sfp , regaining motility. At the end of the crack the quorum communication peptide subtilin triggers a co-ordinated population response from a subtilin-inducible promoter . Upregulating SR1 and rocF promotes arginine and urea production, increasing exogenous CaCO3 deposition. Over-producing yneA induces the filamentous cell phenotype, while SacB converts extracellular sucrose to levansucrose glue.

To protect the environment our project will also include a design for a kill switch.

Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon