Team:Nevada/RD29APromoter

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(rd29A Stress Induced ProAmoter)
(rd29A Stress Induced Promoter)
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<p>From previous studies, the rd29A promoter not only increases the resistance to different stresses in plants, it also minimizes the negative effects such as plant growth reduction (only 30% grown reduction compared to the wild type plant and only a slight growth retardation phenotype on the tobacco plant ) in comparison to the 78% growth reduction of the 35S promoter. This proved that the rd29A is a better promoter than the 35S. Another study also showed that the survival rates of the transgenic clones (with the rd29A promoter) had a greater probability of survival for they recovered after exposure to freezing temperatures that was then returned to normal room temperature. This was compared to the non-transgenic that showed complete damage to the plant with no recovery from freezing. Therefore, the RD29A promoter is critically important for it can potentially improve agricultural techniques that farmers can use for their plants.</p>
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<p>From previous studies, the rd29A promoter not only increases the resistance to different stresses in plants, it also minimizes the negative effects such as plant growth reduction (only 30% grown reduction compared to the wild type plant and only a slight growth retardation phenotype on the tobacco plant ) in comparison to the 78% growth reduction of the 35S promoter. This proved that the rd29A is a better promoter than the 35S. Another study also showed that the survival rates of the transgenic clones (with the rd29A promoter) had a greater probability of survival for they recovered after exposure to freezing temperatures that was then returned to normal room temperature. This was compared to the non-transgenic that showed complete damage to the plant with no recovery from freezing. Therefore, the rd29A promoter is critically important for it can potentially improve agricultural techniques that farmers can use for their plants.</p>
<p>The stress-inducible rd29A (responsive to dehydration 29A) promoter is derived from Arabidopsis thaliana, which is a small flowering plant that is a member of the mustard family (Brassicaceae). DRE and ABA dependent binding sequences have been found within the promoter region, and are shown to be independent of each other. The DRE/CRT binding sequences are the target of the binding protein <html><a href="http://2010.igem.org/Team:Nevada/DREB1CPromoter">DREB1C</a></html>. <html><a href="http://2010.igem.org/Team:Nevada/DREB1CPromoter">DREB1C</a></html> activation of rd29A is relegated by cold stress responses, yet salinity and drought stresses also activate rd29A using alternative ABA independent and dependent transcription factors.</p>
<p>The stress-inducible rd29A (responsive to dehydration 29A) promoter is derived from Arabidopsis thaliana, which is a small flowering plant that is a member of the mustard family (Brassicaceae). DRE and ABA dependent binding sequences have been found within the promoter region, and are shown to be independent of each other. The DRE/CRT binding sequences are the target of the binding protein <html><a href="http://2010.igem.org/Team:Nevada/DREB1CPromoter">DREB1C</a></html>. <html><a href="http://2010.igem.org/Team:Nevada/DREB1CPromoter">DREB1C</a></html> activation of rd29A is relegated by cold stress responses, yet salinity and drought stresses also activate rd29A using alternative ABA independent and dependent transcription factors.</p>

Revision as of 16:03, 25 October 2010


rd29A Stress Induced Promoter

From previous studies, the rd29A promoter not only increases the resistance to different stresses in plants, it also minimizes the negative effects such as plant growth reduction (only 30% grown reduction compared to the wild type plant and only a slight growth retardation phenotype on the tobacco plant ) in comparison to the 78% growth reduction of the 35S promoter. This proved that the rd29A is a better promoter than the 35S. Another study also showed that the survival rates of the transgenic clones (with the rd29A promoter) had a greater probability of survival for they recovered after exposure to freezing temperatures that was then returned to normal room temperature. This was compared to the non-transgenic that showed complete damage to the plant with no recovery from freezing. Therefore, the rd29A promoter is critically important for it can potentially improve agricultural techniques that farmers can use for their plants.

The stress-inducible rd29A (responsive to dehydration 29A) promoter is derived from Arabidopsis thaliana, which is a small flowering plant that is a member of the mustard family (Brassicaceae). DRE and ABA dependent binding sequences have been found within the promoter region, and are shown to be independent of each other. The DRE/CRT binding sequences are the target of the binding protein DREB1C. DREB1C activation of rd29A is relegated by cold stress responses, yet salinity and drought stresses also activate rd29A using alternative ABA independent and dependent transcription factors.


References