Team:Nevada/Agrobacterium Transformations


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Agrobacterium Transformations

Agrobacterium tumefaciens Mediated Transformation of Plant Cells
Agrobacterium tumefaciens

  • Gram (-) soil bacterium
  • Causative agent in crown gall disease
  • Disease is the result of interkingdom transfer of DNA (i.e. bacteria --> plant)

Molecular Progression of Agrobacterium Infection
1. chemical perception of host
2. induction of virulence genes
3. formation of infection apparatus
4. preparation of T-DNA
5. export T-DNA to plant cell
6. import T-DNA to plant nucleus
7. integration of T-DNA into host genome
8. expression of T-DNA

Agrobacterium Transformation"Up Close":

Agrobacterium Transformation Overview: