Team:Nevada/Agrobacterium Transformations

From 2010.igem.org

Revision as of 02:43, 26 October 2010 by Hilarya (Talk | contribs)

Agro header.png

 

Agrobacterium Transformations

Agrobacterium tumefaciens Mediated Transformation of Plant Cells
Agrobacterium tumefaciens

  • Gram (-) soil bacterium
  • Causative agent in crown gall disease
  • Disease is the result of interkingdom transfer of DNA (i.e. bacteria --> plant)


Molecular Progression of Agrobacterium Infection
1. chemical perception of host
2. induction of virulence genes
3. formation of infection apparatus
4. preparation of T-DNA
5. export T-DNA to plant cell
6. import T-DNA to plant nucleus
7. integration of T-DNA into host genome
8. expression of T-DNA