Team:Nevada/Agrobacterium Transformations

From 2010.igem.org

(Difference between revisions)
(Agrobacterium Transformations as an Alternative to NT Cell Transformations)
Line 39: Line 39:
<br>
<br>
<br>
<br>
-
'''Agrobacterium Transformation"Up Close":'''
+
'''Agrobacterium Transformation "Up Close":'''
<br>
<br>
<html><a href="https://static.igem.org/mediawiki/2010/b/be/Up_close_Agro.png"><img src="https://static.igem.org/mediawiki/2010/b/be/Up_close_Agro.png" class="shadow" style="float:center;width:500px;margin:10px"></a>
<html><a href="https://static.igem.org/mediawiki/2010/b/be/Up_close_Agro.png"><img src="https://static.igem.org/mediawiki/2010/b/be/Up_close_Agro.png" class="shadow" style="float:center;width:500px;margin:10px"></a>

Revision as of 03:25, 26 October 2010

Agro header.png

 

Agrobacterium Transformations as an Alternative to NT Cell Transformations

Agrobacterium tumefaciens Mediated Transformation of Plant Cells
Agrobacterium tumefaciens

  • Gram (-) soil bacterium
  • Causative agent in crown gall disease
  • Disease is the result of interkingdom transfer of DNA (i.e. bacteria --> plant)


Molecular Progression of Agrobacterium Infection
1. chemical perception of host
2. induction of virulence genes
3. formation of infection apparatus
4. preparation of T-DNA
5. export T-DNA to plant cell
6. import T-DNA to plant nucleus
7. integration of T-DNA into host genome
8. expression of T-DNA

Agrobacterium Transformation "Up Close":


Agrobacterium Transformation Overview: