Team:Nevada/Agrobacterium Transformations

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(Agrobacterium Transformations)
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<li><a href="http://2010.igem.org/Team:Nevada/BY-2 (NT1)Transformation Protocol" tabindex="1">NT Cell Transformation Protocol</a></li>
<li><a href="http://2010.igem.org/Team:Nevada/BY-2 (NT1)Transformation Protocol" tabindex="1">NT Cell Transformation Protocol</a></li>
<li><a href="http://2010.igem.org/Team:Nevada/Agrobacterium Transformations" tabindex="3">Agrobacterium Transformations</a></li>
<li><a href="http://2010.igem.org/Team:Nevada/Agrobacterium Transformations" tabindex="3">Agrobacterium Transformations</a></li>
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<li><a href="http://2010.igem.org/Team:Nevada/Transgenic Plants" tabindex="3">Transgenic Plants: into the Wild</a></li>
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Revision as of 03:02, 26 October 2010

Agro header.png

 

Agrobacterium Transformations as an Alternative to NT Cell Transformations

Agrobacterium tumefaciens Mediated Transformation of Plant Cells
Agrobacterium tumefaciens

  • Gram (-) soil bacterium
  • Causative agent in crown gall disease
  • Disease is the result of interkingdom transfer of DNA (i.e. bacteria --> plant)


Molecular Progression of Agrobacterium Infection
1. chemical perception of host
2. induction of virulence genes
3. formation of infection apparatus
4. preparation of T-DNA
5. export T-DNA to plant cell
6. import T-DNA to plant nucleus
7. integration of T-DNA into host genome
8. expression of T-DNA

Agrobacterium Transformation"Up Close":


Agrobacterium Transformation Overview: