Team:Nevada/Agrobacterium Transformations

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'''''Agrobacterium tumefaciens'' Mediated Transformation of Plant Cells'''
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''Agrobacterium tumefaciens''
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*Gram (-) soil bacterium
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*Causative agent in crown gall disease
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*Disease is the result of interkingdom transfer of DNA (i.e. bacteria --> plant)
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'''Molecular Progression of Agrobacterium Infection'''
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<br>1. chemical perception of host
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<br>2. induction of virulence genes
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<br>3. formation of infection apparatus
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<br>4. preparation of T-DNA
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<br>5. export T-DNA to plant cell
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<br>6. import T-DNA to plant nucleus
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<br>7. integration of T-DNA into host genome
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<br>8. expression of T-DNA
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Revision as of 02:43, 26 October 2010

Agro header.png

 

Agrobacterium Transformations

Agrobacterium tumefaciens Mediated Transformation of Plant Cells
Agrobacterium tumefaciens

  • Gram (-) soil bacterium
  • Causative agent in crown gall disease
  • Disease is the result of interkingdom transfer of DNA (i.e. bacteria --> plant)


Molecular Progression of Agrobacterium Infection
1. chemical perception of host
2. induction of virulence genes
3. formation of infection apparatus
4. preparation of T-DNA
5. export T-DNA to plant cell
6. import T-DNA to plant nucleus
7. integration of T-DNA into host genome
8. expression of T-DNA