Team:Nevada

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Team Nevada

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You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
The 2010 Nevada iGEM team has three goals for this year’s competition. First, we are going to test the validity of utilizing Nicotiana tabacum protoplasts (NT cells), plant cells without the cell wall, as a model for the expression of higher plant genes for future iGEM competitions. This system is useful in the respect that the time it takes to obtain transgenic lines of cells is greatly shorter than the time to obtain transgenic plants. These cells can therefore be utilized as a quick proof-of-concept test model before moving synthetic constructs into plants of interest. We also aim to produce a plant-specific plasmid, several stress-inducible plant promoters, reporter genes containing Kozak sequences (ribosome binding sites) and terminators that conform to BioBrick standards. Lastly, we hope to measure the induction of these stress promoters in real-time by performing a fluorometry assay in which stress will be applied to NT cells and fluorescent output by a reporter (GFP) will be measured to detail the induction in real time. This method has a distinct advantage over microarray data since microarrays are only one ‘snapshot’ in time.	Your team picture
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We would like to thank the following sponsors for their support in helping us make this project possible. Much thanks to the Departments of Biochemistry and Biotechnology and the College of Agriculture, Biotechnology and Natural Resources for their encouragement and support. Thank you Nevada INBRE for over $6,000 in support for supplies and registration costs. Thank you to Associated Students of the Univeristy of Nevada for support our fund raising efforts. Thank you to Promega Co. for free enzyme donations. Thank you to Invitrogen Co. for a discount on our Vector NTI program.

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-The goal for our project is twofold.  For our project, we will be working with and examining inducible promoters and the timing in which the promoters are expressed.
+The 2010 Nevada iGEM team has three goals for this year’s competition.  First, we are going to test the validity of utilizing Nicotiana tabacum protoplasts (NT cells), plant cells without the cell wall, as a model for the expression of higher plant genes for future iGEM competitions.  This system is useful in the respect that the time it takes to obtain transgenic lines of cells is greatly shorter  than the time to obtain transgenic plants.  These cells can therefore be utilized as a quick proof-of-concept test model before moving synthetic constructs into plants of interest.  We also aim to produce a plant-specific plasmid, several stress-inducible plant promoters, reporter genes containing Kozak sequences (ribosome binding sites) and terminators that conform to BioBrick standards.  Lastly, we hope to measure the induction of these stress promoters in real-time by performing a fluorometry assay in which stress will be applied to NT cells and fluorescent output  by a reporter (GFP) will be measured to detail the induction in real time.  This method has a distinct advantage over microarray data since microarrays are only one ‘snapshot’ in time.''
-We will also be creating several plant parts to be made available for other iGEM teams to use in future competitions.  These parts include a plant specific plasmid, reporter genes with their own Kozak sequences, plant promoters, and plant terminators.''
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