Team:NYU

From 2010.igem.org

(Difference between revisions)
 
(46 intermediate revisions not shown)
Line 1: Line 1:
-
It's NYU's first year at the iGEM competition and we are all synbio newbies, but we are very confident in our project this year! Unlike most other iGEM teams, we are made up of 5 undergraduate students and only two faculty advisers.
+
<html>
-
[[Image:NYU_logo.png|border|250px|right|]]
+
<div id="allcontent">
 +
<img class="bannerimage" src="https://static.igem.org/mediawiki/2010/2/2d/NYU_bannerv_6.png" height="200px" width="950px"><br />
-
'' Our game plan for the international genetically engineered machine competition is to construct a yeast strain capable of independent antibody discovery. Instead of relying on antibody display that require high-throughput fluorescent screening, currently the dominant method for microbial antibody discovery, our strain will be able to select for high antibody binding without outside influence. We will accomplish this by linking the antibody library and antigen with the split ubiquitin system, which will allow the yeast cells to sense the amount of antibody::antigen complexing.  
+
<div id="headerlinks">
-
''
+
<ul id="nav">
 +
<li>
 +
<a class="bannertoplinks" href="https://2010.igem.org/Team:NYU">Home</a>
 +
</li>
 +
 +
<li><a class="bannertoplinks" href="https://2010.igem.org/Team:NYU/Project">Project</a>
 +
<ul style="z-index:1">
 +
<li><a class="bannerlinks" href="https://2010.igem.org/Team:NYU/Project">Overview</a></li>
 +
                        <li><a class="bannerlinks" href="https://2010.igem.org/Team:NYU/Experiments">Experiments</a></li>
 +
                        <li><a class="bannerlinks" href="https://2010.igem.org/Team:NYU/Assembly">Overlap Assembly</a></li>
 +
<li><a class="bannerlinks" href="https://2010.igem.org/Team:NYU/Notebook">Notebook</a></li>
 +
<li><a class="bannerlinks" href="https://2010.igem.org/Team:NYU/Parts">Biobricks</a></li>
 +
<li><a class="bannerlinks" href="https://2010.igem.org/Team:NYU/Programming">Programming</a></li>
 +
</ul>
 +
</li>
 +
<li><a class="bannertoplinks" href="https://2010.igem.org/Team:NYU/Team">Team</a>
 +
                <ul style="z-index:1">
 +
                        <li><a class="bannerlinks" href="https://2010.igem.org/Team:NYU/Team">NYU</a></li>
 +
                        <li><a class="bannerlinks" href="https://2010.igem.org/Team:NYU/CornellMed">Cornell Med</a></li>
 +
                </ul>
 +
</li>
 +
<li><a class="bannertoplinks" href="https://2010.igem.org/Team:NYU/Sponsors">Sponsors</a>
 +
                <ul style="z-index:1">
 +
                        <li><a class="bannerlinks" href="https://2010.igem.org/Team:NYU/Sponsors">Sponsors</a></li>
 +
                        <li><a class="bannerlinks" href="http://www.sciencehouse.com">ScienceHouse</a></li>
 +
                        <li><a class="bannerlinks" href="http://www.nysynbio.org">NY synbio</a></li>
 +
                </ul>
 +
</li>
 +
<li><a class="bannertoplinks" href="https://2010.igem.org/Team:NYU/Contact">Contact Us</a>
 +
             
 +
</li>
 +
</ul>
 +
</div>
 +
</p><p><br />
-
Basically, the single chain variable fragment (scFv) antibody will be fused to the N-terminal domain of ubiquitin (N-ub) and the target antigen will be fused to the C-terminal domain (C-ub).  When the antibody and antigen form a complex, the two domains of ubiquitin are brought together and any protein that is fused downstream of C-ub will be cleaved from the rest of the complex by a ubiquitin protease. To use this mechanism to our advantage, we will fuse the Gal4 activator protein downstream of antigen::C-ub complex. So, when the antibody binds the antigen, Gal4p will be released, translocated into the nucleus and will affect transcription of genes that confer greater cell survival in the environment (either amino acid biosynthesis or, for a control, antibiotic resistance).
+
<style type="text/css">
-
<!--- The Mission, Experiments --->
+
#allcontent {
 +
      width: 950px;
 +
}
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
 
-
!align="center"|[[Team:NYU|Home]]
+
body {
-
!align="center"|[[Team:NYU/Team|Team]]
+
        margin-left: auto;
-
!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=NYU Official Team Profile]
+
        margin-right: auto;
-
!align="center"|[[Team:NYU/Project|Project]]
+
font-family: Helvetica, Arial, sans-serif;
-
!align="center"|[[Team:NYU/Parts|Parts Submitted to the Registry]]
+
width: 900px;
-
!align="center"|[[Team:NYU/Modeling|Modeling]]
+
}
-
!align="center"|[[Team:NYU/Notebook|Notebook]]
+
 
-
!align="center"|[[Team:NYU/Safety|Safety]]
+
table {
 +
        color: #000000;
 +
font-family: Helvetica, Arial, sans-serif;
 +
        }
 +
 
 +
#content {
 +
        color: #000000;
 +
font-family: Helvetica, Arial, sans-serif;
 +
width: 965px;
 +
        line-height: 1.2em;
 +
 
 +
}
 +
 
 +
a {
 +
  text-decoration: none;
 +
  color: #7F5217;
 +
  }
 +
 
 +
a:hover {
 +
color: #A0C544;
 +
        text-decoration: none;
 +
}
 +
 +
h1, h2, h3, h4, h5, h6 {
 +
        color: #254117;
 +
}
 +
 
 +
#headerlinks{
 +
      margin-left: -20px;
 +
}
 +
 
 +
#nav, #nav ul {
 +
padding: 0;
 +
list-style: none;
 +
}
 +
 
 +
#nav a {
 +
display: block;
 +
width: 149px;
 +
}
 +
 
 +
#nav li {
 +
float: left;
 +
width: 149px;
 +
margin-right: 41px;
 +
}
 +
 
 +
#nav li ul {
 +
position: absolute;
 +
width: 149px;
 +
left: -999em;
 +
}
 +
 
 +
#nav li:hover ul {
 +
left: auto;
 +
      text-decoration: none;
 +
      color: white !important;
 +
 
 +
}
 +
 
 +
#nav li:hover ul, #nav li.sfhover ul {
 +
left: auto;
 +
      text-decoration: none;
 +
      color: white !important;
 +
}
 +
 
 +
sfHover = function() {
 +
var sfEls = document.getElementById("nav").getElementsByTagName("LI");
 +
for (var i=0; i<sfEls.length; i++) {
 +
sfEls[i].onmouseover=function() {
 +
this.className+=" sfhover";
 +
}
 +
sfEls[i].onmouseout=function() {
 +
this.className=this.className.replace(new RegExp(" sfhover\\b"), "");
 +
}
 +
}
 +
}
 +
if (window.attachEvent) window.attachEvent("onload", sfHover);
 +
 +
.bannerimage {
 +
 +
}
 +
 
 +
a.bannerlinks {
 +
  color: white !important;
 +
  text-decoration: none;
 +
  }
 +
 
 +
 
 +
a.bannertoplinks {
 +
  color: white !important;
 +
  text-decoration: none;
 +
}
 +
 
 +
a.bannerlinks:hover, a.bannertoplinks:hover {
 +
  background-color: #A0C544;
 +
  color: white;
 +
  }
 +
 +
.bannerlinks {
 +
background-color: #698B69;
 +
border-style: solid;
 +
border-color: white;
 +
border-width: 1px;
 +
color: white;
 +
padding: 3px 20px 3px 20px;
 +
        margin-left: -19px;
 +
}
 +
 
 +
.bannertoplinks {
 +
background-color: #006400;
 +
border-style: solid;
 +
border-color: white;
 +
border-width: 1px;
 +
color: white;
 +
        margin-bottom: -2px;
 +
padding: 3px 20px 3px 20px;
 +
}
 +
 
 +
#sidebar {
 +
width: 300px;
 +
float: right;
 +
margin-right: 0px;
 +
border-style: solid;
 +
border-color: white;
 +
border-width: 1px;
 +
padding: 0px;
 +
        padding-top:5px;
 +
}
 +
 
 +
#abstract {
 +
width: 600px;
 +
border-style: solid;
 +
border-color: white;
 +
border-width: 1px;
 +
padding: 10px;
 +
line-height: 1.2em;
 +
        font-size: 14px;
 +
}
 +
 +
 +
#rightgroup {
 +
float: right;
 +
width: 300px;
 +
padding: 10px;
 +
}
 +
 +
#leftgroup {
 +
width: 300px;
 +
padding: 10px;
 +
        margin-right: 20px;
 +
}
 +
 +
#maincontent {
 +
width: 610px;
 +
}
 +
 
 +
#headerlinks > #nav > li {
 +
        border-bottom: 4px solid white;
 +
}
 +
 
 +
#sidebar a{
 +
      text-decoration: none;
 +
      color: #7F5217;
 +
}
 +
 
 +
#sidebar a:hover{
 +
    color: #A0C544;
 +
    text-decoration: none;
 +
}
 +
 
 +
a.sidebarlinks {
 +
display: block;
 +
width: 300px;
 +
font-size: 1.2em;
 +
text-align: center;
 +
background-color: #827B60;
 +
color: white !important;
 +
padding: 10px 0px 10px 0px;
 +
margin-bottom: 5px
 +
}
 +
 
 +
a.sidebarlinks:hover {
 +
background-color: #A0C544;
 +
        color: white !important;
 +
}
 +
 
 +
a.timelinelinks {
 +
      color: #7F5217 !important;
 +
}
 +
 
 +
a.timelinelinks:hover {
 +
      color: #A0C544 !important;
 +
}
 +
 
 +
 
 +
</style>
 +
<!-- !END NYU CSS- Thanks Harvard -->
 +
 
 +
</html>
 +
 
 +
 
 +
<div id="container"><div id="header">
 +
 
 +
<html>
 +
 
 +
<script language="JavaScript">
 +
TargetDate = "11/06/2010 12:00 AM UTC-0500";
 +
BackColor = "white";
 +
ForeColor = "green";
 +
CountActive = true;
 +
CountStepper = -1;
 +
LeadingZero = true;
 +
DisplayFormat = "%%D%% Days, %%H%% Hours, %%M%% Minutes and %%S%% Seconds until the iGEM Jamboree!";
 +
FinishMessage = "iGEM time!!";
 +
</script>
 +
<script language="JavaScript" src="http://scripts.hashemian.com/js/countdown.js"></script>
 +
 
 +
 
 +
<style type="text/css">
 +
<!--
 +
 
 +
body
 +
{
 +
font-family: Trebuchet MS, Arial, Helvetica, sans-serif;
 +
color: green;
 +
}
 +
 
 +
td,th {
 +
    color: white;
 +
}
 +
 
 +
table
 +
{
 +
background-color: white;
 +
font-color: green;
 +
color: white;
 +
}
 +
 
 +
 
 +
 
 +
#bodyContent
 +
{
 +
background-color: white;
 +
color: green;
 +
}
 +
 
 +
#content
 +
{
 +
background-color: white;
 +
color: green;
 +
}
 +
 
 +
#footer-box
 +
{
 +
background-color: linen;
 +
color: green;
 +
}
 +
 
 +
p
 +
{
 +
background-color: white;
 +
color: green;
 +
}
 +
 
 +
body
 +
{
 +
background-color: white;
 +
font-color: green;
 +
}
 +
 
 +
-->
 +
</style></head>
 +
</style>
 +
<html>
 +
<body>
 +
<map name="Map" id="Map">
 +
<area shape="rect" coords="3,-1,76,35" href="https://2010.igem.org/Team:NYU" alt="Home" />
 +
<area shape="rect" coords="78,-1,156,39" href="https://2010.igem.org/Team:NYU/Project" alt="Project" />
 +
<area shape="rect" coords="158,0,247,39" href="https://2010.igem.org/Team:NYU/Notebook" alt="Notebook" />
 +
<area shape="rect" coords="248,0,342,34" href="https://2010.igem.org/Team:NYU/Programming" alt="Programming" />
 +
<area shape="rect" coords="343,-1,430,38" href="https://2010.igem.org/Team:NYU/Parts" alt="BioBricks" />
 +
<area shape="rect" coords="432,2,559,36" href="https://2010.igem.org/Team:NYU/Safety" alt="Safety" />
 +
<area shape="rect" coords="562,2,713,36" href="https://2010.igem.org/Team:NYU/Sponsors" alt="Sponsors" />
 +
<area shape="rect" coords="718,-1,862,38" href="https://2010.igem.org/Team:NYU/Team" alt="Team" />
 +
<area shape="rect" coords="854,0,966,38" href="https://2010.igem.org/Team:NYU/Contacts" alt="Contacts" />
 +
</map>
 +
</a>
 +
</body>
 +
</html>
 +
 
 +
 
 +
</div></div><div id="sidebar1">
 +
{|
 +
|-valign="center" border="0"
 +
|width="250px"|
 +
<html>
 +
<style type="text/css">
 +
<!--
 +
body,td,th {
 +
color: green;
 +
    font-weight: bold;
 +
}
 +
a {
 +
color: green;
 +
}
 +
a {
 +
color: green;
 +
text-decoration: none;
 +
}
 +
-->
 +
</style>
 +
<h3 align="center"><a href="https://2010.igem.org/Team:NYU/Parts">Biobricks</a></h3>
 +
</html>
 +
 
 +
----
 +
<html>
 +
<center><a href="https://2010.igem.org/Team:NYU/Parts"><img src="https://static.igem.org/mediawiki/2010/9/97/StandardAssemblyTwoIntoOne.png" width="200" height="180" alt="biobricks" /></a></center>
 +
</html>
 +
 
 +
 
 +
 
 +
<html>
 +
<style type="text/css">
 +
<!--
 +
body,td,th {
 +
color: green;
 +
    font-weight: bold;
 +
}
 +
a {
 +
color: green;
 +
}
 +
a {
 +
color: green;
 +
text-decoration: none;
 +
}
 +
-->
 +
</style>
 +
<h3 align="center"><a href="https://2010.igem.org/Team:NYU/Sponsors">Our Sponsors</a></h3>
 +
</html>
 +
----
 +
<html>
 +
<center><a href="https://2010.igem.org/Team:NYU/Sponsors"><img src="https://static.igem.org/mediawiki/2010/f/ff/Sponsornyu.jpg" width="200" height="180" alt="sponsors" /></a></center>
 +
</html>
 +
 
 +
 
 +
|width="500px"|
 +
 
 +
 
 +
<html>
 +
<style type="text/css">
 +
<!--
 +
body,td,th {
 +
color: green;
 +
    font-weight: bold;
 +
}
 +
a {
 +
color: green;
 +
}
 +
a {
 +
color: green;
 +
text-decoration: none;
 +
}
 +
-->
 +
</style>
 +
<h2 align="center"><a href="https://2010.igem.org/Team:NYU/Notebook">Notebook</a></h2>
 +
</html>
 +
<html>
 +
<center><a href="https://2010.igem.org/Team:NYU/Notebook"><img src="https://static.igem.org/mediawiki/2010/0/0f/NYU_Note3.png"  width="200" height="180" alt="notebook" /></a></center>
 +
</html>
 +
 
 +
 
 +
<html>
 +
<style type="text/css">
 +
<!--
 +
body,td,th {
 +
color: green;
 +
    font-weight: bold;
 +
}
 +
a {
 +
color: green;
 +
}
 +
a {
 +
color: green;
 +
text-decoration: none;
 +
}
 +
-->
 +
</style>
 +
<h1 align="center"><a href="https://2010.igem.org/Team:NYU/Project">The Project</a></h1>
 +
</html>
 +
<html>
 +
<center><a href="https://2010.igem.org/Team:NYU/Project"><img src="https://static.igem.org/mediawiki/2010/d/d8/NYU_logo.png" width="300" height="280"  alt="project" /></a></center>
 +
</html>
 +
 
 +
 
 +
 
 +
<html>
 +
<style type="text/css">
 +
<!--
 +
body,td,th {
 +
color: green;
 +
    font-weight: bold;
 +
}
 +
a {
 +
color: green;
 +
}
 +
a {
 +
color: green;
 +
text-decoration: none;
 +
}
 +
-->
 +
</style>
 +
<h2 align="center"><a href="https://2010.igem.org/Team:NYU/Programming">Programming</a></h2>
 +
</html>
 +
<html>
 +
<center><a href="https://2010.igem.org/Team:NYU/Programming"><img src="https://static.igem.org/mediawiki/2010/2/24/Computer.JPG" width="200" height="140"  alt="Programming" /></a></center>
 +
</html>
 +
 
 +
|width="250px"|
 +
 
 +
<html>
 +
<style type="text/css">
 +
<!--
 +
body,td,th {
 +
color: green;
 +
    font-weight: bold;
 +
}
 +
a {
 +
color: green;
 +
}
 +
a {
 +
color: green;
 +
text-decoration: none;
 +
}
 +
-->
 +
</style>
 +
<h3 align="center"><a href="https://2010.igem.org/Team:NYU/Team">Team</a></h3>
 +
</html>
 +
----
 +
<html>
 +
<center><a href="https://2010.igem.org/Team:NYU/Team"><img src="https://static.igem.org/mediawiki/2010/5/53/Hockey-team-1890.jpg"  width="200" height="180"  alt="team" /></a></center>
 +
</html>
 +
 
 +
 
 +
 
 +
<html>
 +
<style type="text/css">
 +
<!--
 +
body,td,th {
 +
color:green;
 +
    font-weight: bold;
 +
}
 +
a {
 +
color: green;
 +
}
 +
a {
 +
color: green;
 +
text-decoration: none;
 +
}
 +
-->
 +
</style>
 +
<h3 align="center"><a href="https://2010.igem.org/Team:NYU/Safety">Safety</a></h3>
 +
</html>
 +
----
 +
<html>
 +
<center><a href="https://2010.igem.org/Team:NYU/Safety"><img src="https://static.igem.org/mediawiki/2010/d/d8/Fire-blanket-sign.jpg"  width="200" height="180"  alt="safety" /></a></center>
 +
</html>
|}
|}
 +
 +
 +
<div>
 +
 +
 +
 +
 +
 +
</div>

Latest revision as of 09:14, 27 October 2010




Biobricks


biobricks


Our Sponsors


sponsors



Notebook

notebook


The Project

project


Programming

Programming

Team


team


Safety


safety