Team:Michigan/Oil Sands August September
From 2010.igem.org
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'''Transformation of the Flu Operon + Kan backbone ligation''' | '''Transformation of the Flu Operon + Kan backbone ligation''' | ||
- | The [[Media:Protocol_Heat_Shock_Transformation_2.pdf|Heat shock transformation protocol]] was used | + | The [[Media:Protocol_Heat_Shock_Transformation_2.pdf|Heat shock transformation protocol]] was used. |
- | ''E.Coli | + | ''E.Coli DH5α'' was used as competent cells and had a prewashing OD600 of .845 and a post washing OD600 of .787. |
- | Two | + | Two separate transformations were performed both with 100 uL Comp. Cells + 10 uL Flu + KAN BB Ligation. |
Both transformations were plated on LB+Kan+IPTG(30 uL) and are currently incubating at 37 C in the ERB. | Both transformations were plated on LB+Kan+IPTG(30 uL) and are currently incubating at 37 C in the ERB. |
Revision as of 03:56, 26 October 2010