Team:Michigan/Oil Sands August September
From 2010.igem.org
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*Lane 8: uncut pBAD (loaded 1 uL of miniprep into gel) | *Lane 8: uncut pBAD (loaded 1 uL of miniprep into gel) | ||
- | We did not get the pBAD part | + | We did not get the pBAD part due to the miniprep culture overgrowing and the cells lysing their chromosomal DNA (resulting in extremely long bands in the gel). We will try miniprepping the DNA from this culture again letting the culture grow out for a shorter amount of time before the miniprep. |
'''flu primers''' | '''flu primers''' | ||
- | Primers were ordered today to amplify the flu operon of E. coli K12 by colony PCR | + | Primers were ordered today to amplify the flu operon of E. coli K12 by colony PCR: |
*[[Media:7-31-2010_Flu_operon_primers.pdf|General primer design]] | *[[Media:7-31-2010_Flu_operon_primers.pdf|General primer design]] |
Revision as of 03:30, 26 October 2010