Team:Michigan/Oil Sands
From 2010.igem.org
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*pBAD #1 was digested with EcoRI and SpeI | *pBAD #1 was digested with EcoRI and SpeI | ||
*pBAD #2 was digested with SpeI and PstI | *pBAD #2 was digested with SpeI and PstI | ||
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+ | ==8/2/2010== | ||
'''Gel of Digested Parts''' | '''Gel of Digested Parts''' | ||
+ | |||
+ | [[Image:7-30-2010_Biofilm_formation_in_LB_media_jpeg.jpg|500px]] | ||
+ | |||
+ | *Lane 1: invitrogen 1 kb plus ladder | ||
+ | *Lane 2: GFP #3 cut with XbaI and PstI (GFP: 720 bp Backbone: 2079 bp) | ||
+ | *Lane 3: GFP #4 cut with EcoRI and XbaI | ||
+ | *Lane 4: uncut GFP plamid (only 1 uL left so did not show up on gel) | ||
+ | *Lane 5: pBAD cut with EcoRI and SpeI(pBAD: 1210 bp backbone: 4425 bp) | ||
+ | *Lane 6: pBAD cut with SpeI and PstI | ||
+ | *Lane 7: uncut pBAD (loaded very small amount into gel by accident) | ||
+ | *Lane 8: uncut pBAD (loaded 1 uL of miniprep into gel) |
Revision as of 17:10, 2 August 2010