Team:Michigan/Oil Sands
From 2010.igem.org
(Difference between revisions)
(→7/26/2010) |
|||
Line 253: | Line 253: | ||
==7/27/2010== | ==7/27/2010== | ||
+ | |||
+ | '''Biobrick Transformation of suface display and pBAD''' | ||
+ | |||
+ | ''Ann'' | ||
+ | |||
+ | Transformation Results | ||
+ | |||
+ | The GFP and linker transformation worked and many colonies grew out on the 100 mg/mL AMP plates | ||
+ | |||
+ | The pBAD part did not grow out on the KAN plates. Upon further inspection this plasmid needs to be grown with IPTG to switch the origin of replication to a high copy above 100 copies per cell. Otherwise, another orgin of replication dominates that is less than 10 copies per cell and the antibiotic concentration of 50 mg/mL maybe too high. This transformation will have to be tried again | ||
+ | |||
+ | At 9:00am a 5 mL culture of the newly transformed GFP part, the newly transformed linker part, the ompA part transformed previously and the INP from the UC davis team were started for a miniprep in LB with 100 mg/mL AMP. | ||
+ | |||
+ | At 9:00pm the GFP, linker and INP parts were miniprepped. The ompA culture had not grown out (b/c not enough -80C freezer stock was added) and a new culture was restarted at 11:00pm in 5 mL of LB with 100 mg/mL AMP. | ||
+ | |||
+ | Pouring Plates | ||
+ | |||
+ | More LB with 100 mg/mL AMP plates and LB with 25 mg/mL KAN plates were poured | ||
+ | |||
==7/28/2010== | ==7/28/2010== | ||
==7/29/2010== | ==7/29/2010== | ||
==7/30/2010== | ==7/30/2010== |
Revision as of 00:42, 31 July 2010