Team:Michigan/Oil Sands
From 2010.igem.org
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|[[Team:Michigan/Oil_Sands#7/29/2010|7/29/2010]] | |[[Team:Michigan/Oil_Sands#7/29/2010|7/29/2010]] | ||
|[[Team:Michigan/Oil_Sands#7/30/2010|7/30/2010]] | |[[Team:Michigan/Oil_Sands#7/30/2010|7/30/2010]] | ||
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30 uL of 1M IPTG solution was added to a 25 mg/mL KAN plate and spread with sterile beads in order to keep the high copy plasmid in the pBAD part while plating | 30 uL of 1M IPTG solution was added to a 25 mg/mL KAN plate and spread with sterile beads in order to keep the high copy plasmid in the pBAD part while plating | ||
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+ | ==7/30/2010== | ||
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+ | '''Transformation of pBAD''' | ||
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+ | The transformation was successful and there were about 30 colonies on the plate. One colony was picked at 7:30 pm for a miniprep the next day | ||
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+ | Another culture was started of GFP from the -80C freezer stock. Both the ligations with GFP did not work, so as long as we are miniprepping and digesting we want to re-verify this part |
Revision as of 14:31, 1 August 2010