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Team:Michigan/Oil Sands
From 2010.igem.org
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**The positive and negative controls grew out accordingly | **The positive and negative controls grew out accordingly | ||
**Only BBa_179015 and BBa_179005 had colonies (both from plate 1 and none from plate 2...) | **Only BBa_179015 and BBa_179005 had colonies (both from plate 1 and none from plate 2...) | ||
| - | ***BBa_179015 had | + | ***BBa_179015 had over 100 colonies |
| - | ***BBa_179005 had | + | ***BBa_179005 had 2 colonies |
*5 mL overnight cultures were started with 100 mg/mL amp at 8pm from a single colony on each plate | *5 mL overnight cultures were started with 100 mg/mL amp at 8pm from a single colony on each plate | ||
Revision as of 15:28, 13 July 2010
| Sunday | Monday | Tuesday | Wednesday | Thursday | Friday | Saturday | |
| Week 1 | - | 6/28/2010 | 6/29/2010 | 6/30/2010 | 7/1/2010 | 7/2/2010 | - |
| Week 2 | - | - | - | 7/7/2010 | 7/8/2010 | 7/9/2010 | 7/10/2010 |
| Week 3 | - | - | - | - | - | - | - |
Oil Sands Lab Notebook
This team includes Ann Lesnefsky, Bryce Rajabian, and Kilho Lee.
6/28/2010
Pseudomonas putida KT2440 Antibiotic Resistance
- Protocol for starting overnight culture from -80°C freezer
- All protocols can also be found in the Protocols section of the Notebook
6/29/2010
Pseudomonas putida KT2440 Antibiotic Resistance
6/29/2010 and 6/30/2010
Pseudomonas putida KT2440 Antibiotic Resistance
7/1/2010
Literature Review Napthenic Acids Composition & HPLC Analysis
7/2/2010
Pseudomonas putida KT2440 Antibiotic Resistance
7/7/2010
Ann
Biobrick Transformation with Alex and Jennifer
- Made CaCl2 solution, and 100 mg/mL amphicilin stock solution according to the media section of the wiki
- Started an overnight culture of DH5alpha according to the heat shock transformation protocol
- A 12 mL culture was started because we were multiplying the protocol by 4
7/8/2010
Ann
Biobrick Transformation with Marc, Katie and Audra according to the heat shock transformation protocol
- Started culture for biobrick transformation from over night at 2:30
- At 5:00pm the cultures had overgrown to an OD600 of 1.2
- A a 1:3 dilution of cells was performed and the cells were allowed to go through another doubling period of 20 minutes
- The OD600 was measured again and found to be around 0.500
- the cultures were placed on ice at 5:30 and allowed to chill for 20 minutes
- The washings were performed and the comp cells were resuspended in the residual CaCl2 only
- The OD600 of the comp cells without the glycerol was above 0.3 therefore the comp cells were concentrated enough
- The heat shock was performed for the following biobricks
- BBa_179015
- BBa_179005
- BBa_145001
- BBa_117008
- BBa_117002
- BBa_103006
- The cultures were placed in the 30C shaker to grow up for an hour at 30C at 9:00pm
- The cultures were plated at 10:00pm
7/9/2010
Ann
Biobrick Transformation with Josh, Prae, Charlie according to the miniprep protocol
- After 9 hours of growing at 37C, the plates did not have any colonies on them
- After 22 hours of growth plates that had grown had clear colonies
- The positive and negative controls grew out accordingly
- Only BBa_179015 and BBa_179005 had colonies (both from plate 1 and none from plate 2...)
- BBa_179015 had over 100 colonies
- BBa_179005 had 2 colonies
- 5 mL overnight cultures were started with 100 mg/mL amp at 8pm from a single colony on each plate
7/10/2010
Ann
Biobrick Transformation with Marcus, Bryce, Kilho, Josh, Charlie, Jeremy Miniprep
- Made frozen stocks from overnight culture of miniprep
- Performed miniprep on BBa_ and BBa_
- Measured DNA concentration with the Nanodrop
- BBa_
- BBa_
