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| - | {|style="color:#1c2bf2;background-color:#fafa19;font-size:9pt;text-align:center" cellpadding="5" cellspacing="0" border="1" bordercolor="#fff" width="62%"
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| - | !
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| - | |Sunday
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| - | |Monday
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| - | |Tuesday
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| - | |Wednesday
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| - | |Thursday
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| - | |Friday
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| - | |Saturday
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| - | !Week 1
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| - | |[[Team:Michigan/Oil_Sands#6/28/2010|6/28/2010]]
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| - | |[[Team:Michigan/Oil_Sands#6/29/2010|6/29/2010]]
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| - | |[[Team:Michigan/Oil_Sands#6/29/2010 and 6/30/2010|6/30/2010]]
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| - | |[[Team:Michigan/Oil_Sands#7/1/2010|7/1/2010]]
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| - | |[[Team:Michigan/Oil_Sands#7/2/2010|7/2/2010]]
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| - | !Week 2
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| - | |[[Team:Michigan/Oil_Sands#7/7/2010|7/7/2010]]
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| - | |[[Team:Michigan/Oil_Sands#7/8/2010|7/8/2010]]
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| - | |[[Team:Michigan/Oil_Sands#7/9/2010|7/9/2010]]
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| - | |[[Team:Michigan/Oil_Sands#7/10/2010|7/10/2010]]
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| - | !Week 3
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| - | |[[Team:Michigan/Oil_Sands#7/12/2010|7/12/2010]]
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| - | |[[Team:Michigan/Oil_Sands#7/13/2010|7/13/2010]]
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| - | |[[Team:Michigan/Oil_Sands#7/14/2010|7/14/2010]]
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| - | |}
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| - | ==Oil Sands Lab Notebook==
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| | | | |
| - | This team includes [[User:annlesn|Ann Lesnefsky]], [[User:rajabiab|Bryce Rajabian]], and [[User:kilholee|Kilho Lee]].
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| - | ==6/28/2010== | + | ==Oil Sands Lab Notebook== |
| - | ''' ''Pseudomonas putida'' KT2440 Antibiotic Resistance'''
| + | |
| - | *[[Media:6-28-2010_Protocol_for_making_cultures_from_a_-80C_freezer_stock.pdf|Protocol for starting overnight culture from -80°C freezer]]
| + | |
| - | *All protocols can also be found in the ''Protocols'' section of the Notebook
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| - | | + | |
| - | ==6/29/2010==
| + | |
| - | ''' ''Pseudomonas putida'' KT2440 Antibiotic Resistance'''
| + | |
| - | *[[Media:6-29-2010_Making_Frozen_Stoks.pdf|Protocol for making frozen stocks]]
| + | |
| - | | + | |
| - | ==6/29/2010 and 6/30/2010==
| + | |
| - | ''' ''Pseudomonas putida'' KT2440 Antibiotic Resistance'''
| + | |
| - | *[[Media:6-27-2010_P._putida_antibiotic_resistance_protocol.pdf|Protocol for ''P. putida'' KT2440 antibotic resistance]]
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| - | | + | |
| - | ==7/1/2010==
| + | |
| - | ''' ''Literature Review'' Napthenic Acids Composition & HPLC Analysis'''
| + | |
| - | *[[Media:6-29-2010_Naphthenic_acids_Composition.pdf|Literature Review of the composition of napthenic acids in oil sands]]
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| - | | + | |
| - | ==7/2/2010==
| + | |
| - | *[[Media:6-30-10_HPLC_Column_Comparison_Sheet1.pdf|Literature Review of HPLC columns for analysis of napthenic acids]]
| + | |
| - | ''' ''Pseudomonas putida'' KT2440 Antibiotic Resistance'''
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| - | *[[Media:7-2-2010_Results_P._putida_antibiotic_resistance.pdf|Results]]
| + | |
| - | ==7/7/2010==
| + | |
| - | ''Ann''
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| - | | + | |
| - | '''Biobrick Transformation''' with Alex and Jennifer
| + | |
| - | *Made CaCl2 solution, and 100 mg/mL amphicilin stock solution according to the media section of the wiki
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| - | *Started an overnight culture of DH5alpha according to the heat shock transformation protocol
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| - | **A 12 mL culture was started because we were multiplying the protocol by 4
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| - | | + | |
| - | ==7/8/2010==
| + | |
| - | ''Ann''
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| - | | + | |
| - | '''Biobrick Transformation''' with Marc, Katie and Audra according to the heat shock transformation protocol
| + | |
| - | *Started culture for biobrick transformation from over night at 2:30
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| - | *At 5:00pm the cultures had overgrown to an OD600 of 1.2
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| - | **A a 1:3 dilution of cells was performed and the cells were allowed to go through another doubling period of 20 minutes
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| - | *The OD600 was measured again and found to be around 0.500
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| - | *the cultures were placed on ice at 5:30 and allowed to chill for 20 minutes
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| - | *The washings were performed and the comp cells were resuspended in the residual CaCl2 only
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| - | *The OD600 of the comp cells without the glycerol was above 0.3 therefore the comp cells were concentrated enough
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| - | *The heat shock was performed for the following biobricks
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| - | **BBa_179015
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| - | **BBa_179005
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| - | **BBa_145001
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| - | **BBa_117008
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| - | **BBa_117002
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| - | **BBa_103006
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| - | *The cultures were placed in the 30C shaker to grow up for an hour at 30C at 9:00pm
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| - | *The cultures were plated at 10:00pm
| + | |
| - | ==7/9/2010==
| + | |
| - | ''Ann''
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| - | | + | |
| - | '''Biobrick Transformation''' with Josh, Prae, Charlie according to the miniprep protocol
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| - | *After 9 hours of growing at 37C, the plates did not have any colonies on them
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| - | *After 22 hours of growth plates that had grown had clear colonies
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| - | **The positive and negative controls grew out accordingly
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| - | **Only BBa_179015 and BBa_179005 had colonies (both from plate 1 and none from plate 2...)
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| - | ***BBa_179015 had over 100 colonies
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| - | ***BBa_179005 had 2 colonies
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| - | *5 mL overnight cultures were started with 100 mg/mL amp at 8pm from a single colony on each plate
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| - | | + | |
| - | ==7/10/2010==
| + | |
| - | ''Ann''
| + | |
| - | | + | |
| - | '''Biobrick Transformation''' with Marcus, Bryce, Kilho, Josh, Charlie, Jeremy
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| - | | + | |
| - | Miniprep
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| - | *Made frozen stocks from overnight culture of miniprep
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| - | *Performed miniprep on BBa_179015 and BBa_179005
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| - | *Measured DNA concentration with the Nanodrop
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| - | **BBa_179015-11 ng/mL
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| - | **BBa_179005-40 ng/mL
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| - | | + | |
| - | Digest
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| - | *Ran according to the digest protocol in the protocol section
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| - | **Cut parts with EcoRI and PstI
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| - | *Digested at 37C for 30 minutes
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| - | | + | |
| - | Gel
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| - | *Ran according to the protocol in the protocol section
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| - | *Used 8 well comb instead of 13 well comb so the samples were too dilute too see
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| - | | + | |
| - | ==7/12/2010==
| + | |
| - | | + | |
| - | ''Ann''
| + | |
| - | | + | |
| - | '''Biobrick Transformation''' with Jeremy
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| - | | + | |
| - | Gel
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| - | | + | |
| - | [[Image:7-12-2010_T7_promoter_and_T7_gfp_biobricks.JPG|300px]]
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| - | *Reran gel from 7/10/2010
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| - | **Lane 1-Invitrogen 1 kb plus ladder
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| - | **Lane 2-Digested Bba_179015
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| - | ***Ran out of undigested Bba_179015
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| - | **Lane 3-Digested Bba_179005
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| - | **Lane 4-Uncut miniprep plasmid Bba_179005
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| - | | + | |
| - | We found that we successfully extracted BBa_1790XX, T7-GFP, because there were expected bands at 906 and 2079 as faintly seen in the gel picture above. No appeared for biobrick Bba_1790XX, T7 promoter, so this biobrick will be transformed again.
| + | |
| - | | + | |
| - | '''Biobrick Transformation take 2''' with Jeremy
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| - | | + | |
| - | Autoclaved DI water for transformation and autoclaved sterile containers
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| - | *to autoclave sterile containers fill with DI water and decant the water before you start the culture in the container
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| - | | + | |
| - | ==7/13/2010==
| + | |
| - | | + | |
| - | '''Biobrick Transformation take 2''' with Jeremy, Marcus, Josh, Kevin, Audra, Katie
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| - | | + | |
| - | ''Ann''
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| - | | + | |
| - | Performed according to the electroporation transformation
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| - | *Started the culture at 9:05am
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| - | *Removed the culture at 12:05pm with an OD600 of 0.809
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| - | *The OD600 of the comp cells were 1.2 after washing
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| - | *The time constant for all electroporation were between 5.6 and 5.8
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| - | *The cultures were allowed to grow in the incubator from 2:15-4:00pm
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| - | **The cultures started to clump after growing this time
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| - | *100 uL of cells were plated on 100 mg/mL AMP plates
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| - | | + | |
| - | ==7/14/2010==
| + | |
| - | | + | |
| - | '''Biobrick Transformation take 2'''
| + | |
| | | | |
| - | ''Ann''
| + | Members of this team include [[User:annlesn|Ann Lesnefsky]], [[User:rajabiab|Bryce Rajabian]], [[User:chillywings|Marcus Lehr]], [[User:Infekt|Alex Pyden]], [[User:Jeremigo|Jeremy Golden]], [[User:Seongkyu|John Seong Kyu Yang]] and [[User:kilholee|Kilho Lee]]. |
| | | | |
| - | Electroporation Transformation
| + | ==Monthly Notebooks== |
| | + | Notebooks are ordered by month. |
| | | | |
| - | All of the transformation plates grew out! (minus the negative control of course)
| + | [[Team:Michigan/Oil_Sands_June_July | June/July]] |
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| - | This means we should do all transformations by electroporation from now on. Just check with the Lin Lab to make sure we can use the electroporation machine for a few hours before starting
| + | [[Team:Michigan/Oil_Sands_August_September | August/September]] |
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| - | Miniprep
| + | [[Team:Michigan/Oil_Sands_October | October]] |
| - | *Started overnight cultures in 5 mL of LB plus 100 mg/mL AMP
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| - | **The following biobricks were started from the transformation plate
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| - | **The following biobricks were started from the frozen stock in the -80C freezer
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| | | | |
| - | INPNC
| + | =='''In the Lab'''== |
| - | *We received the INPNC biobrick from UC Davis 2009 team. THANK YOU!!!
| + | <html> |
| - | *It was shipped on LB plates in a pMA-SK plasmid from Mr. Gene in ''E. coli'' DH5alpha
| + | <iframe src="http://www-personal.umich.edu/~shanwu/wiki_photos/src/themes/classic/classic-demo.html" width="100%" height="650px" scrolling="no"></iframe> |
| | + | </html> |
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