Team:Michigan/Oil Sands

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{{Michigan Header}}
{{Michigan Header}}
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!
 
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|Sunday
 
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|Monday
 
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|Thursday
 
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!Week 1
 
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|[[Team:Michigan/Oil_Sands#6/28/2010|6/28/2010]]
 
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|[[Team:Michigan/Oil_Sands#6/29/2010|6/29/2010]]
 
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|[[Team:Michigan/Oil_Sands#6/29/2010 and 6/30/2010|6/30/2010]]
 
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|[[Team:Michigan/Oil_Sands#7/1/2010|7/1/2010]]
 
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|[[Team:Michigan/Oil_Sands#7/2/2010|7/2/2010]]
 
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!Week 2
 
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|[[Team:Michigan/Oil_Sands#7/7/2010|7/7/2010]]
 
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|[[Team:Michigan/Oil_Sands#7/8/2010|7/8/2010]]
 
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|[[Team:Michigan/Oil_Sands#7/9/2010|7/9/2010]]
 
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|[[Team:Michigan/Oil_Sands#7/10/2010|7/10/2010]]
 
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!Week 3
 
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|[[Team:Michigan/Oil_Sands#7/12/2010|7/12/2010]]
 
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==Oil Sands Lab Notebook==
 
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This team includes [[User:annlesn|Ann Lesnefsky]], [[User:rajabiab|Bryce Rajabian]], and [[User:kilholee|Kilho Lee]].
 
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==6/28/2010==
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==Oil Sands Lab Notebook==
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''' ''Pseudomonas putida'' KT2440 Antibiotic Resistance'''
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*[[Media:6-28-2010_Protocol_for_making_cultures_from_a_-80C_freezer_stock.pdf|Protocol for starting overnight culture from -80°C freezer]]
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*All protocols can also be found in the ''Protocols'' section of the Notebook
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==6/29/2010==
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''' ''Pseudomonas putida'' KT2440 Antibiotic Resistance'''
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*[[Media:6-29-2010_Making_Frozen_Stoks.pdf|Protocol for making frozen stocks]]
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==6/29/2010 and 6/30/2010==
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''' ''Pseudomonas putida'' KT2440 Antibiotic Resistance'''
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*[[Media:6-27-2010_P._putida_antibiotic_resistance_protocol.pdf|Protocol for ''P. putida'' KT2440 antibotic resistance]]
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==7/1/2010==
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''' ''Literature Review'' Napthenic Acids Composition & HPLC Analysis'''
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*[[Media:6-29-2010_Naphthenic_acids_Composition.pdf|Literature Review of the composition of napthenic acids in oil sands]]
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==7/2/2010==
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*[[Media:6-30-10_HPLC_Column_Comparison_Sheet1.pdf|Literature Review of HPLC columns for analysis of napthenic acids]]
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''' ''Pseudomonas putida'' KT2440 Antibiotic Resistance'''
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*[[Media:7-2-2010_Results_P._putida_antibiotic_resistance.pdf|Results]]
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==7/7/2010==
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''Ann''
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'''Biobrick Transformation''' with Alex and Jennifer
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*Made CaCl2 solution, and 100 mg/mL amphicilin stock solution according to the media section of the wiki
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*Started an overnight culture of DH5alpha according to the heat shock transformation protocol
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**A 12 mL culture was started because we were multiplying the protocol by 4
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==7/8/2010==
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''Ann''
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'''Biobrick Transformation''' with Marc, Katie and Audra according to the heat shock transformation protocol
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*Started culture for biobrick transformation from over night at 2:30
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*At 5:00pm the cultures had overgrown to an OD600 of 1.2
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**A a 1:3 dilution of cells was performed and the cells were allowed to go through another doubling period of 20 minutes
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*The OD600 was measured again and found to be around 0.500
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*the cultures were placed on ice at 5:30 and allowed to chill for 20 minutes
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*The washings were performed and the comp cells were resuspended in the residual CaCl2 only
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*The OD600 of the comp cells without the glycerol was above 0.3 therefore the comp cells were concentrated enough
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*The heat shock was performed for the following biobricks
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**BBa_179015
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**BBa_179005
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**BBa_145001
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**BBa_117008
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**BBa_117002
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**BBa_103006
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*The cultures were placed in the 30C shaker to grow up for an hour at 30C at 9:00pm
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*The cultures were plated at 10:00pm
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==7/9/2010==
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''Ann''
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'''Biobrick Transformation''' with Josh, Prae, Charlie according to the miniprep protocol
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*After 9 hours of growing at 37C, the plates did not have any colonies on them
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*After 22 hours of growth plates that had grown had clear colonies
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**The positive and negative controls grew out accordingly
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**Only BBa_179015 and BBa_179005 had colonies (both from plate 1 and none from plate 2...)
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***BBa_179015 had over 100 colonies
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***BBa_179005 had 2 colonies
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*5 mL overnight cultures were started with 100 mg/mL amp at 8pm from a single colony on each plate
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==7/10/2010==
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''Ann''
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'''Biobrick Transformation''' with Marcus, Bryce, Kilho, Josh, Charlie, Jeremy
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Miniprep
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*Made frozen stocks from overnight culture of miniprep
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*Performed miniprep on BBa_179015 and BBa_179005
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*Measured DNA concentration with the Nanodrop
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**BBa_179015-11 ng/mL
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**BBa_179005-40 ng/mL
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Digest
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*Ran according to the digest protocol in the protocol section
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**Cut parts with EcoRI and PstI
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*Digested at 37C for 30 minutes
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Gel
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*Ran according to the protocol in the protocol section
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*Used 8 well comb instead of 13 well comb so the samples were too dilute too see
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==7/12/2010==
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Members of this team include [[User:annlesn|Ann Lesnefsky]], [[User:rajabiab|Bryce Rajabian]], [[User:chillywings|Marcus Lehr]], [[User:Infekt|Alex Pyden]], [[User:Jeremigo|Jeremy Golden]], [[User:Seongkyu|John Seong Kyu Yang]] and [[User:kilholee|Kilho Lee]].
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''Ann''
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==Monthly Notebooks==
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Notebooks are ordered by month.
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'''Biobrick Transformation''' with Jeremy
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[[Team:Michigan/Oil_Sands_June_July | June/July]]
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Gel
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[[Team:Michigan/Oil_Sands_August_September | August/September]]
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*Reran gel from 7/10/2010
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[[Image:File.jpg]]  
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[[Team:Michigan/Oil_Sands_October | October]]
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**Lane 1-Invitrogen 1 kb plus ladder
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=='''In the Lab'''==
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**Lane 2-Digested Bba_179015
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<html>
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***Ran out of undigested Bba_179015
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<iframe src="http://www-personal.umich.edu/~shanwu/wiki_photos/src/themes/classic/classic-demo.html" width="100%" height="650px" scrolling="no"></iframe>
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**Lane 3-Digested Bba_179005
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</html>
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**Lane 4-Uncut miniprep plasmid Bba_179005
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Latest revision as of 00:05, 27 October 2010


Michigan Header





Oil Sands Lab Notebook

Members of this team include Ann Lesnefsky, Bryce Rajabian, Marcus Lehr, Alex Pyden, Jeremy Golden, John Seong Kyu Yang and Kilho Lee.

Monthly Notebooks

Notebooks are ordered by month.

June/July

August/September

October

In the Lab

Retrieved from "http://2010.igem.org/Team:Michigan/Oil_Sands"