Team:Michigan/Media Recipes

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Media Recipes

LB media

  • 10 g LB broth
  • 500 mL DI water

autoclave to sterilize

LB media with agar

  • 10 g LB broth
  • 7.5 g agar
  • 500 mL DI water

autoclave to sterilize

add antibiotics after autoclaving when warm to touch (about 50°C)

pour plates when warm to the touch (about 50°C)

0.1 M CaCl2

  • X g of CaCl2
  • X mL of DI water

filter sterilize

Ampicillin Antibiotic Stock Solution (100 mg/mL)

  • 1 g of ampicillin
  • 5 mL of DI water
  • 5 mL of ethanol

filter sterilize and make 1 mL aliquotes to store in the -20°C freezer

Kanamycin Antibiotic Stock Solution (50 mg/mL)

  • 250 mg of kanamycin
  • 5 mL of DI water

filter sterilize and make 1 mL aliquots to store in the -20°C freezer

Tetracycline Antibiotic Stock Solution (50 mg/mL)

  • 250 mg of tetracycline
  • 2.5 mL of DI water
  • 2.5 mL of ethanol

filter sterilize and make 1 mL aliquots to store in the -20°C freezer