"
Team:Mexico-UNAM-CINVESTAV/Notebook/Week Two
From 2010.igem.org
(Difference between revisions)
| Line 20: | Line 20: | ||
==''Tuesday''== | ==''Tuesday''== | ||
| - | ==='''Transformed Top 10 competent cells, and did 10 plates incubated overnight'''=== | + | ==='''Transformed Top 10 competent cells, and did 10 plates incubated overnight.'''=== |
==''Wednsday''== | ==''Wednsday''== | ||
| - | ==='''Results previous day transformations only two plates were completed | + | ==='''Results previous day transformations only two plates were completed unsucessful.'''=== |
| - | + | ||
*'''Prepared solutions for miniprep via Alcalin lisis method''' | *'''Prepared solutions for miniprep via Alcalin lisis method''' | ||
[[Image:Imagen2.gif]] | [[Image:Imagen2.gif]] | ||
| + | |||
| + | |||
| + | ==''Thursday''== | ||
| + | |||
| + | ==='''The inoculum for miniprep we prepared 6 vector’s vials'''=== | ||
| + | ==='''without positive results. Maybe the solutions for Alcaline'''=== | ||
| + | ==='''lisis are not well.'''=== | ||
| + | |||
| + | [[Image:Imagen3.gif ]] | ||
Revision as of 04:27, 24 October 2010
Contents |
Week 2 13th September - 17th 2010 Holidays in México
Week 3 18th September -22th 2010
Monday
Planned and prepared for tomorrow's electroporation
- Depart electroporator.
- Made up LB agar plates and cloramphenicol 100μl.
- Plan to transform Psb1C3 froma plate.
We were looking for a nanodrop to quantify DNA’s concentrations
and tried to make ligations Psb1C3 with each one of primers
(our this year's bricks).
Tuesday
Transformed Top 10 competent cells, and did 10 plates incubated overnight.
Wednsday
Results previous day transformations only two plates were completed unsucessful.
- Prepared solutions for miniprep via Alcalin lisis method
Thursday
The inoculum for miniprep we prepared 6 vector’s vials
without positive results. Maybe the solutions for Alcaline
lisis are not well.
