Team:Mexico-UNAM-CINVESTAV/Notebook/Week Two

From 2010.igem.org

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(Replacing page with '{{Mexico-UNAM-CINVESTAV-HEADER}} = Week 2 13th September - 17th 2010 Holidays in México =')
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= Week 2  13th  September - 17th  2010 Holidays in México =
= Week 2  13th  September - 17th  2010 Holidays in México =
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= Week 3  18th September -22th 2010 =
 
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==''Monday''==
 
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==='''Planned and prepared for tomorrow's electroporation'''===
 
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*'''Depart electroporator.'''
 
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*'''Made up LB agar plates and cloramphenicol 100μl.'''
 
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*'''Plan to transform Psb1C3 froma plate.'''
 
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==='''We were looking for a nanodrop to quantify DNA’s concentrations'''===
 
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==='''and tried to make ligations Psb1C3 with each one of primers'''===
 
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==='''(our this year's bricks).'''===
 
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==''Tuesday''==
 
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==='''Transformed Top 10 competent cells, and did 10 plates incubated overnight.'''===
 
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==''Wednsday''==
 
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==='''Results previous day transformations only two plates were completed unsucessful.'''===
 
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*'''Prepared solutions for miniprep via Alcalin lisis method'''
 
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[[Image:Imagen2.gif]]
 
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==''Thursday''==
 
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==='''The inoculum for miniprep we prepared 6 vector’s vials'''=== 
 
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==='''without positive results. Maybe the solutions for alcaline'''===
 
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==='''lisis are not well.'''===
 
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[[Image:Imagen3.gif|500px]]
 
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*'''We have discused over how to quantify our cold sistem response'''
 
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'''we gonig to use a promoter sistem, the funcional casset  using  J13002  as follow'''
 
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*'''Promoter + our functional secuence + GFP'''
 
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[[Image:Cuant1.gif]]
 
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*'''Promoter + our functional secuence + AFP (Anty freeze protein)
 
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[[Image:Imagen4.gif]]
 
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*'''We have a meeting with our Genomics frend’s we going to get a
 
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'''colaboration on Modeling and probably at wet lab too.
 

Revision as of 05:52, 24 October 2010


Week 2 13th September - 17th 2010 Holidays in México