Team:Mexico-UNAM-CINVESTAV/Notebook/Week Six

From 2010.igem.org

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==''Wednesday''==
==''Wednesday''==
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 +
'''We ran a agarosa's gel to extract from the band E0240 and I20260.'''
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{| border="1"
 +
|-
 +
|DNA
 +
|50μl
 +
|-
 +
|Tango
 +
|9μl
 +
|-
 +
|EcoRI
 +
|5μl
 +
|-
 +
|PstI
 +
|5μl
 +
|-
 +
|H2O
 +
|6μl
 +
|-
 +
|Total
 +
|75μl
 +
|}

Revision as of 06:47, 27 October 2010


Week #6

11th October - 15 October 2010

Monday

We kept inoculated cells on LB liquid medium at 37ºC overnight, each containing 30ml.

DSC02090.JPG


Prepared miniprep for I20260 and E0240, we are going to work on the characterize the promoter

and the cold expresion casstte by RPU's.


Biobricks002.jpg



Tuesday

Yesterday's overnight inoculum we did miniprep and double digestion with EcoRI and PstI

To confirm the ligation of PCR 4.


DNA 10μl
Tango 1.8μl
EcoRI 1μl
PstI 1μl
H2O 1.20μl
Total 15μl


PCR4.gif


Wednesday

We ran a agarosa's gel to extract from the band E0240 and I20260.


DNA 50μl
Tango 9μl
EcoRI 5μl
PstI 5μl
H2O 6μl
Total 75μl