Team:Mexico-UNAM-CINVESTAV/Notebook/Week Seven

From 2010.igem.org

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==''Monday''==
==''Monday''==
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'''We prepared inoculum 6 falcon tubes of  E0240 and I20260 each, after'''
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'''We prepared inoculum in 6 falcon tubes of  E0240 and I20260 each, after'''  
-
 
+
-
'''nano espectophotometer Readings we are going to need huge amounts of plasmid'''
+
 +
'''nano espectophotometer readings we are going to need huge amounts of plasmid'''
==''Tuesday''==
==''Tuesday''==
 +
'''With yesterday's inoculum we prepared six vials of culture to do miniprep and extracted from the band'''
-
==''Wednsday''==
+
'''we ran a agarosa's gel with 16μl per well, then precipitated with salts and alcohol the DNA and kept
 +
 
 +
'''overnight at -20°C''''
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 +
 
 +
[[Image:Biobricks013.jpg|400px]]
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 +
 
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==''Wednesday''==
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'''We made the ligation with the one hour amresco kit and transformed in 4 plates'''
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 +
'''Aditional to this tried to ligate with Fermentas T4 this reaction is overnight at 4°'''
==''Thursday''==
==''Thursday''==
 +
'''We had transformant cells'''
 +
 +
[[Image:DSC02335.gif|400px]]
 +
 +
'''We are goingo to use the previous ligations to caracterise our biobricks.'''
==''Friday''==
==''Friday''==
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'''We send our biobricks good luck lab team'''
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[[Image:DSC02329.gif|400px]]

Latest revision as of 23:23, 27 October 2010



Week #7

18th October - 22th October 2010

Monday

We prepared inoculum in 6 falcon tubes of E0240 and I20260 each, after

nano espectophotometer readings we are going to need huge amounts of plasmid

Tuesday

With yesterday's inoculum we prepared six vials of culture to do miniprep and extracted from the band

we ran a agarosa's gel with 16μl per well, then precipitated with salts and alcohol the DNA and kept

overnight at -20°C'


Biobricks013.jpg


Wednesday

We made the ligation with the one hour amresco kit and transformed in 4 plates

Aditional to this tried to ligate with Fermentas T4 this reaction is overnight at 4°


Thursday

We had transformant cells

DSC02335.gif

We are goingo to use the previous ligations to caracterise our biobricks.

Friday

We send our biobricks good luck lab team


DSC02329.gif