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From 2010.igem.org

As summer project our experimental work begin in August. After intensive brain-storming we finnaly decide between two options.

* Inmunoresponse using Synthetic Biology 

* Criobiology applied to plant crops

After a selection process we are going to choose the Criobiology Project

The final design of our expresions moduls for the project were as follow.

Next Notebook paper is our reference for primers using, ligations and moduls Assembly.

The AFP (Antifreeze protein) was synthesized as below

Week #1

06th September - 10th September 2010

Monday

After discution we conclude the Igem’s vector (vial with green cover) is not enough.

First step transform only the vector to get enough and begin ligations.

We are going to transform Psb1C3 from plate. For this:

• Prepare a stock of Cloramphenicol, Kanamicyn, Ampicilin solutions.

• Make LB agar plates.

• Complete procedure for making quimio and electro-competent cells.

Tuesday

Completed competent cells stored aliquots at -80 degrees each vial with 150μl.

We transformed TOP10 cells with Psb1C3.

• Planned and prepared for tomorrow's transformation

Wednesday

For a strange razon we have not transformats cells

today we are going to check out the Cloramphenicol

dose and try again the transformation with Psb1C3.

Thursday

In vector’s absence we have recived the primer’s synthesis

and proceed to amplify by PCR.

The amplification is correct and we have to look for a nanodrop

to quantify DNA’s concentrations.