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Team:Mexico-UNAM-CINVESTAV/Notebook/Week One
From 2010.igem.org
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| - | '''Next | + | '''Next Notebook paper is our reference for primers using, ligations and moduls Assembly.''' |
[[Image:Primers1.JPG |center|500px|]] | [[Image:Primers1.JPG |center|500px|]] | ||
| - | '''The AFP ( | + | '''The AFP (Antifreeze protein) was synthesized as below''' |
[[Image:Vector001.jpg|center|500px|]] | [[Image:Vector001.jpg|center|500px|]] | ||
| Line 35: | Line 35: | ||
==='''After discution we conclude the Igem’s vector (vial with green cover) is not enough.'''=== | ==='''After discution we conclude the Igem’s vector (vial with green cover) is not enough.'''=== | ||
| - | ==='''First step transform only the vector to get | + | ==='''First step transform only the vector to get enough and begin ligations.'''=== |
| - | ==='''We going to transform Psb1C3 from plate. For this:'''=== | + | ==='''We are going to transform Psb1C3 from plate. For this:'''=== |
| Line 44: | Line 44: | ||
*''' Make LB agar plates. ''' | *''' Make LB agar plates. ''' | ||
| - | *'''Complete procedure for making quimio and electro competent | + | *'''Complete procedure for making quimio and electro-competent cells.''' |
| Line 58: | Line 58: | ||
==='''For a strange razon we have not transformats cells'''=== | ==='''For a strange razon we have not transformats cells'''=== | ||
| - | ==='''today we going to check out the Cloramphenicol'''=== | + | ==='''today we are going to check out the Cloramphenicol'''=== |
==='''dose and try again the transformation with Psb1C3.'''=== | ==='''dose and try again the transformation with Psb1C3.'''=== | ||
==''Thursday''== | ==''Thursday''== | ||
| - | ==='''In vector’s absence we have recived the primer’s | + | ==='''In vector’s absence we have recived the primer’s synthesis'''=== |
==='''and proceed to amplify by PCR.'''=== | ==='''and proceed to amplify by PCR.'''=== | ||
[[Image:PCR.gif]] | [[Image:PCR.gif]] | ||
| - | ==='''The amplification is correct and we have to | + | ==='''The amplification is correct and we have to look for a nanodrop'''=== |
==='''to quantify DNA’s concentrations.'''=== | ==='''to quantify DNA’s concentrations.'''=== | ||
Revision as of 05:47, 26 October 2010
As summer project our experimental work begin in August. After intensive brain-storming we finnaly decide between two options.
* Inmunoresponse using Synthetic Biology
* Criobiology applied to plant crops
After a selection process we are going to choose the Criobiology Project
The final design of our expresions moduls for the project were as follow.
.JPG)
Next Notebook paper is our reference for primers using, ligations and moduls Assembly.
The AFP (Antifreeze protein) was synthesized as below
Week #1
06th September - 10th September 2010
Monday
After discution we conclude the Igem’s vector (vial with green cover) is not enough.
First step transform only the vector to get enough and begin ligations.
We are going to transform Psb1C3 from plate. For this:
- Prepare a stock of Cloramphenicol, Kanamicyn, Ampicilin solutions.
- Make LB agar plates.
- Complete procedure for making quimio and electro-competent cells.
Tuesday
Completed competent cells stored aliquots at -80 degrees each vial with 150μl.
We transformed TOP10 cells with Psb1C3.
- Planned and prepared for tomorrow's transformation
Wednesday
For a strange razon we have not transformats cells
today we are going to check out the Cloramphenicol
dose and try again the transformation with Psb1C3.
Thursday
In vector’s absence we have recived the primer’s synthesis
and proceed to amplify by PCR.
