Team:Mexico-UNAM-CINVESTAV/Notebook/Week One

From 2010.igem.org

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'''As summer proyect our experimental work began at agust after intensive brian storming finaly deccided between two options.'''
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'''As summer project our experimental work begin in August. After intensive brain-storming we finnaly decide between two options.'''
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  '''* Inmunoresponse using Syintetic biology and
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  '''* Inmunoresponse using Synthetic Biology
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  '''* Cryobiology applied to plant tissues.
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  '''* Criobiology applied to plant crops
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= '''After selection proces we going to go  with a Cryobiology Proyect''' =
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= '''After a selection process we are going to choose the Criobiology Project''' =
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'''The final design our expresion’s moduls for the poryect were as follow.'''
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'''The final design of our expresions moduls for the project were as follow.'''
[[Image:Primers(2).JPG |center|500px||border|caption]]
[[Image:Primers(2).JPG |center|500px||border|caption]]

Revision as of 05:04, 26 October 2010



As summer project our experimental work begin in August. After intensive brain-storming we finnaly decide between two options.

* Inmunoresponse using Synthetic Biology 
* Criobiology applied to plant crops

After a selection process we are going to choose the Criobiology Project

The final design of our expresions moduls for the project were as follow.

caption


Next Notebook paper is our reference for primers using, ligations and moduls Assambly.


Primers1.JPG

The AFP (Anty freeze protein) was synthesized as below

Vector001.jpg


Week #1

06th September - 10th September 2010

Monday

After discution we conclude the Igem’s vector (vial with green cover) is not enough.

First step transform only the vector to get enough and begin ligations.

We going to transform Psb1C3 from plate. For this:

  • Prepare a stock of Cloramphenicol, Kanamicyn, Ampicilin solutions.
  • Make LB agar plates.
  • Complete procedure for making quimio and electro competent cels.


Tuesday

Completed competent cells stored aliquots at -80 degrees each vial with 150μl.

We transformed TOP10 cells with Psb1C3.

  • Planned and prepared for tomorrow's transformation

Wednesday

For a strange razon we have not transformats cells

today we going to check out the Cloramphenicol

dose and try again the transformation with Psb1C3.

Thursday

In vector’s absence we have recived the primer’s sintesis

and proceed to amplify by PCR.

PCR.gif

The amplification is correct and we have to looking for a nanodrop

to quantify DNA’s concentrations.