"
Team:Mexico-UNAM-CINVESTAV/Notebook/Week Four
From 2010.igem.org
Contents |
Week 4 25th September - 29th 2010
Monday
Well we are stuck with the vector this week we have to get vector is our main objetive.
- We have achived a nanodrop readings as below
| ng/μl | 260/280 | 260/230 | |
| PsB1C3 | 0.60 | -0.73 | -0.0 |
| PCR 1 red | 427.70 | 1.71 | 1.71 |
| PCR 2 red | 483.20 | 1.74 | 1.71 |
| PCR 3 red | 410.70 | 1.76 | 2.02 |
| PCR 4 red | 431.05 | 1.61 | 1.88 |
| PCR 1 blue | 533.35 | 1.71 | 1.75 |
| PCR 2 blue | 536.00 | 1.72 | 1.82 |
| PCR 3 blue | 577.50 | 1.69 | 1.59 |
| PCR 4 blue | 627.55 | 1.70 | 1.56 |
| PsB1C3 | 4.10 | 2.62 | 1.01 |
- Yep our trouble is the lisis alcaline method to do the mini prep
we have a low concentration of vector’s plasmid DNA.
We have to work an try to get more plasmid and make dilutions of PCR’s
is not posible to do ligations with this ratio betwen vector an insert.
On this step we advisor has proposed a method via Low Meelting agarose
to geting out plasmid from the band.
Tuesday
We run with low meelting’s to extract band after this we did
a protocol to precipit with alchol an salts our objetive is do evrething
to get vector and do the ligations.
