Team:Macquarie Australia/Notebook3
From 2010.igem.org
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+ | <h3><big> Figure 10. Qiagen Plasmid Midi Prep results: </big></h3> | ||
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+ | <b> Figure 10. </b> GelRed post-stained 1.5% agarose gel of pET-3A Plasmid purification. In lanes 1, 4, 5 and 15 there is a 1kb Molecular ladder. In lane 2 there is the Plasmid purification sample A and in lane 3 is the Plasmid purification sample B. In lanes 6 to 14 there are flow-throughs that were collected at different stages throughout the protocol. There is some DNA visible in these lanes so some of the DNA has been lost. Possibly genomic DNA they are quite high (didn’t move through the wells)</p><p> | ||
Revision as of 09:31, 24 October 2010
PROJECT LAB BOOK
Welcome to the Macquarie University project lab book page!
DAY-BY-DAY PROGRESS FOR THE TRANSFORMATION AND CLONING
22nd September 2010
Plasmid Prep using Qiagen Plasmid Midi Prep Kit
Figure 10. Qiagen Plasmid Midi Prep results:
Figure 10. GelRed post-stained 1.5% agarose gel of pET-3A Plasmid purification. In lanes 1, 4, 5 and 15 there is a 1kb Molecular ladder. In lane 2 there is the Plasmid purification sample A and in lane 3 is the Plasmid purification sample B. In lanes 6 to 14 there are flow-throughs that were collected at different stages throughout the protocol. There is some DNA visible in these lanes so some of the DNA has been lost. Possibly genomic DNA they are quite high (didn’t move through the wells)