Team:MIT tchara

From 2010.igem.org

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<dt><b>Bacteria</b></dt>
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<dd>
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<ul>
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                        <li><a href="https://2010.igem.org/Team:MIT_toggle">Overview</a></li>
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<li><a href="https://2010.igem.org/Team:MIT_tconst">Toggle Construction</a></li>
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<li><a href="#">Characterization</a></li>
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</ul>
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</dd>
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<dl id="nav">
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<dt><b>Phage</b></dt>
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<dd>
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<ul>
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<li><a href="https://2010.igem.org/Team:MIT_phage">Introduction</a></li>
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<li><a href="https://2010.igem.org/Team:MIT_phage_background">Background</a></li>
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<li><a href="https://2010.igem.org/Team:MIT_phage_design">Design</a></li>
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<li><a href="https://2010.igem.org/Team:MIT_phage_construction">Construction</a></li>
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<li><a href="https://2010.igem.org/Team:MIT_phage_results">Results</a></li>
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<li><a href="https://2010.igem.org/Team:MIT_phage_context">Context</a></li>
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</ul>
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</dd>
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<dt><b>Mammalian</b></dt>
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<dd>
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<ul>
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                        <li><a href="https://2010.igem.org/Team:MIT_mammalian">Overview</a></li>
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<li><a href="https://2010.igem.org/Team:MIT_mammalian_Standard">New Mammalian Standard </a></li>
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                        <li><a href="https://2010.igem.org/Team:MIT_mammalian_Circuit">Circuit Design</a></li>
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<li><a href="https://2010.igem.org/Team:MIT_mammalian_Mechanosensation"> Mechanosensation</a></li>
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<li><a href="https://2010.igem.org/Team:MIT_mammalian_Bone"> Bone Formation</a></li>
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<li><a href="https://2010.igem.org/Team:MIT_mammalian_Switch"> Synthetic Switch</a></li>
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</ul>
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</dd>
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</dl>
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<div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;">
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<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">the bacterial uv toggle</div></td>
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<tr><td><br>In the beginning, there was a UV Toggle
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(<a href="http://www.pnas.org/content/101/22/8414.full">Kobayashi/Collins, 2004</a>). <br> <br>The 2010 MIT iGEM team saw that it was good, and decided to implement the Collins toggle in E.coli to create cells with bistable phenotypes.
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We planned for the toggle to create patterned fluorescence and phage polymerization in response to exposing the cells to UV light. <br><br>
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<div class="outline">
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<a href="#first">1 First Circuit</a><br>
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&nbsp;&nbsp;&nbsp;&nbsp;<a href="#collins">1.1 Collins Toggle Validation</a><br>
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&nbsp;&nbsp;&nbsp;&nbsp;<a href="#biobrick">1.2 First Biobrick</a><br>
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&nbsp;&nbsp;&nbsp;&nbsp;<a href="#improved">1.3 Improved Bacterial Camera</a><br>
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<a href="#ahl">2 AHL Propagation</a><br>
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&nbsp;&nbsp;&nbsp;&nbsp;<a href="#ahlconst">2.1 Construction</a><br>
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&nbsp;&nbsp;&nbsp;&nbsp;<a href="#ahlmovie">2.2 Movie</a><br>
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<a href="#lpt">3 Low Power Toggle</a><br>
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&nbsp;&nbsp;&nbsp;&nbsp;<a href="#lptconst">3.1 Construction</a><br>
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&nbsp;&nbsp;&nbsp;&nbsp;<a href="#lptimproved">3.2 Improved Toggle</a><br>
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<a href="#toggles">4 Biobricking the Toggles</a><br>
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<a href="#improving">5 Improving R0065</a><br>
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</div></td><tr><td><br>

Revision as of 22:23, 27 October 2010

Bacteria
the bacterial uv toggle

In the beginning, there was a UV Toggle (Kobayashi/Collins, 2004).

The 2010 MIT iGEM team saw that it was good, and decided to implement the Collins toggle in E.coli to create cells with bistable phenotypes. We planned for the toggle to create patterned fluorescence and phage polymerization in response to exposing the cells to UV light.

1 First Circuit
    1.1 Collins Toggle Validation
    1.2 First Biobrick
    1.3 Improved Bacterial Camera
2 AHL Propagation
    2.1 Construction
    2.2 Movie
3 Low Power Toggle
    3.1 Construction
    3.2 Improved Toggle
4 Biobricking the Toggles
5 Improving R0065

Retrieved from "http://2010.igem.org/Team:MIT_tchara"