Team:MIT results

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<div class="bodybaby">Results</div></td>
<div class="bodybaby">Results</div></td>
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<strong>Bacteria</strong><br>
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<strong>Bacteria - Toggle and Circuit</strong><br>
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In E.Coli, the MIT Bacterial team endeavored to design a system of UV-inducible polymerizing phage. In order to establish and fine-tune the control of phage growth, we focused on implementing and improving the Collins toggle, a bistable genetic switch in E.Coli. The UV power required to switch the Collins toggle to the "on" state was killing many of our cells. To counteract this cell death, we biobricked a Low Power Toggle that required 1/8 the UV power to switch states. Another problem we faced was in a leaky hybrid promoter R0065. It would create basal signal in some cells that were not supposed to be in the "off" state. We improved this hybrid promoter so that it is more tightly controlled by our input. <a href="http://2010.igem.org/Team:MIT_tconst"><b>See the results! &rarr;</b></a><br><br>
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The MIT Bacterial team endeavored to implement a system of UV-inducible polymerizing phage in E. Coli.  To establish the necessary fine control of phage growth, we focused on implementing and improving the Collins toggle, a bistable genetic switch in E.Coli. The UV power required to switch the Collins toggle to the "on" state was killing many of our cells. To counteract this cell death, we designed and constructed a modified toggle that required eight times less UV power to switch states. Another problem we encountered was the high level of leakiness of the Plux/CI hybrid promoter R0065. We designed and constructed an improved version of this hybrid promoter that is more tightly controlled by our input. <a href="http://2010.igem.org/Team:MIT_tconst"><b>See the results! &rarr;</b></a><br><br>
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<strong>Phage</strong><br>
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<strong>Phage - Biomaterial Formation</strong><br>
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The Phage team is well on its way to making hairy cells that express polymerizing phage.
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The Phage team is well on its way to making hairy cells that express polymerizing phage. We have observed the poly-phage phenotype with AFM.  We have displayed various linker proteins on the phage coat.  We have constructed a new chloramphenicol pBR322 derived biobrick backbone to allow co-transformation of our final circuit with both our toggle (SC101, Amp) and hyperphage (p15a, Kan).  <a href="http://2010.igem.org/Team:MIT_phage_results"><b>See the results! &rarr;</b></a><br><br>

Revision as of 02:11, 28 October 2010

Results

Bacteria - Toggle and Circuit
The MIT Bacterial team endeavored to implement a system of UV-inducible polymerizing phage in E. Coli. To establish the necessary fine control of phage growth, we focused on implementing and improving the Collins toggle, a bistable genetic switch in E.Coli. The UV power required to switch the Collins toggle to the "on" state was killing many of our cells. To counteract this cell death, we designed and constructed a modified toggle that required eight times less UV power to switch states. Another problem we encountered was the high level of leakiness of the Plux/CI hybrid promoter R0065. We designed and constructed an improved version of this hybrid promoter that is more tightly controlled by our input. See the results! →

Phage - Biomaterial Formation
The Phage team is well on its way to making hairy cells that express polymerizing phage. We have observed the poly-phage phenotype with AFM. We have displayed various linker proteins on the phage coat. We have constructed a new chloramphenicol pBR322 derived biobrick backbone to allow co-transformation of our final circuit with both our toggle (SC101, Amp) and hyperphage (p15a, Kan). See the results! →