Team:MIT psb6c5

From 2010.igem.org

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From the <a href="http://2010.igem.org/Team:MIT"><b>2010 MIT iGEM Team</b></a>.<a style="float:right;" href="http://partsregistry.org/Part:pSB6C5"><b>See the Parts Registry Page!! &rarr;</b></a><br><br>
From the <a href="http://2010.igem.org/Team:MIT"><b>2010 MIT iGEM Team</b></a>.<a style="float:right;" href="http://partsregistry.org/Part:pSB6C5"><b>See the Parts Registry Page!! &rarr;</b></a><br><br>
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<b class="bolded">Plasmid Backbone from Reshma's design</b><br>
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<b class="bolded">pBR322 Derived Chloramphenicol Backbone</b><br>
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The biobrick backbone K415049 was constructed using the standard biobrick <a href="http://partsregistry.org/Help:Plasmids/Construction">base vector method</a> developed by Reshma Shetty et al.  The pMB1 replication origin for pBR322 is a regulated version of the high copy pUC19 origin.  The copy number is expected to be around 20.  Due to homologies between the K415049 and the high copy pUC19 based vectors, there is an naturally occurring reverse sequencing site within the origin and regulatory regions.  Thus, K415049 has an extra VR site.  As of the wiki freeze (10/27/10), we are still characterizing the copy number and working on removing the extra sequencing site.
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pSB6C5 would be the revised version of K415049 with the extra reverse sequencing site removed.  A PCR based strategy has been developed for removing the extraneous region.
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<a href="http://2010.igem.org/wiki/images/1/17/K415049.jpg" class="thickbox"><img width=100% src="http://2010.igem.org/wiki/images/1/17/K415049.jpg"></a><br>
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<a href="http://2010.igem.org/wiki/images/4/4f/PSB6C5.jpg" class="thickbox"><img width=100% src="http://2010.igem.org/wiki/images/4/4f/PSB6C5.jpg"></a><br>
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Vector maps created with Geneious v5.
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Latest revision as of 15:28, 27 October 2010

pSB6C5


From the 2010 MIT iGEM Team.See the Parts Registry Page!! →

pBR322 Derived Chloramphenicol Backbone
The biobrick backbone K415049 was constructed using the standard biobrick base vector method developed by Reshma Shetty et al. The pMB1 replication origin for pBR322 is a regulated version of the high copy pUC19 origin. The copy number is expected to be around 20. Due to homologies between the K415049 and the high copy pUC19 based vectors, there is an naturally occurring reverse sequencing site within the origin and regulatory regions. Thus, K415049 has an extra VR site. As of the wiki freeze (10/27/10), we are still characterizing the copy number and working on removing the extra sequencing site.

pSB6C5 would be the revised version of K415049 with the extra reverse sequencing site removed. A PCR based strategy has been developed for removing the extraneous region.


Vector maps created with Geneious v5.

← pSB1K16All Backbones →