Team:MIT phage construction

From 2010.igem.org

(Difference between revisions)
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Components: RBS : M13 PVIII</li>
Components: RBS : M13 PVIII</li>
<li>
<li>
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>Explanation: This is a basic translational unit: a ribosome-binding site and the p8 gene.  You can attach any promoter in front of it for expression.</li></ul>
+
Explanation: This is a basic translational unit: a ribosome-binding site and the p8 gene.  You can attach any promoter in front of it for expression.</li></ul>
<br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png">
<br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png">
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<br><img src="https://static.igem.org/mediawiki/2010/4/4a/138.png">
<br><img src="https://static.igem.org/mediawiki/2010/4/4a/138.png">
<br><br><b>BBa_K415147</b>
<br><br><b>BBa_K415147</b>
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<br>Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-Fos
+
<ul><li>
-
<br>Explanation:
+
Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-Fos/li>
 +
<li>Explanation:
 +
</li></ul>
<br><img src="">
<br><img src="">
<br><br><b>BBa_K415148</b>
<br><br><b>BBa_K415148</b>
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<br>Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-Jun
+
<ul><li>
-
<br>Explanation:
+
Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-Jun</li>
 +
<li>Explanation:
 +
</li></ul>
<br><img src="">
<br><img src="">
<br><br><b>BBa_K415149</b>
<br><br><b>BBa_K415149</b>
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<br>Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-ACID
+
<ul><li>
-
<br>Explanation:
+
Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-ACID/li>
 +
<li>Explanation:
 +
</li></ul>
<br><img src="">
<br><img src="">
<br><br><b>BBa_K415150</b>
<br><br><b>BBa_K415150</b>
-
<br>Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-BASE
+
<ul><li>
-
<br>Explanation:
+
Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-BASE</li>
 +
<li>Explanation:
 +
</li></ul>
<br><img src="">
<br><img src="">
<br><br><b>BBa_K415151</b>
<br><br><b>BBa_K415151</b>
-
<br>Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-GR1
+
<ul><li>
-
<br>Explanation:
+
Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-GR1</li>
 +
<li>Explanation:
 +
</li></ul>
<br><img src="">
<br><img src="">
<br><br><b>BBa_K415152</b>
<br><br><b>BBa_K415152</b>
-
<br>Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-GR2
+
<ul><li>
-
<br>Explanation:
+
Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-GR2</li>
 +
<li>Explanation:
 +
</li></ul>
<br><img src="">
<br><img src="">
</td>
</td>

Revision as of 23:24, 24 October 2010

hairy cells and polymerizing phage - construction

PARTS

BBa_K415100
  • Components: RBS : M13 PVIII
  • Explanation: This is a basic translational unit: a ribosome-binding site and the p8 gene. You can attach any promoter in front of it for expression.



BBa_K415108
  • Components: RBS : M13 PIII
  • Explanation: This is a basic translational unit: a ribosome-binding site and the p3 gene. You can attach any promoter in front of it for expression.



BBa_K415138
  • Components: pLac : RBS : M13 PIII
  • Explanation: This is a basic expression unit: a promoter in front of a ribosome-binding site and the p3 gene. We used this to generate constitutive expression of p3 in cells infected with hyperphage in an attempt to rescue the wild-type, non-hairy phenotype. See the results page for an AFM image of this working.



BBa_K415147
  • Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-Fos/li>
  • Explanation:



BBa_K415148
  • Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-Jun
  • Explanation:



BBa_K415149
  • Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-ACID/li>
  • Explanation:



BBa_K415150
  • Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-BASE
  • Explanation:



BBa_K415151
  • Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-GR1
  • Explanation:



BBa_K415152
  • Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-GR2
  • Explanation:

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