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Team:MIT phage construction
From 2010.igem.org
(Difference between revisions)
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<br><b><a href="http://partsregistry.org/Part:BBa_K415100">BBa_K415100</a></b> | <br><b><a href="http://partsregistry.org/Part:BBa_K415100">BBa_K415100</a></b> | ||
<ul><li> | <ul><li> | ||
| - | + | Components: RBS : M13 PVIII</li> | |
<li> | <li> | ||
| - | + | >Explanation: This is a basic translational unit: a ribosome-binding site and the p8 gene. You can attach any promoter in front of it for expression.</li></ul> | |
<br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png"> | <br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png"> | ||
<br><br><b><a href="http://partsregistry.org/Part:BBa_K415108">BBa_K415108</a></b> | <br><br><b><a href="http://partsregistry.org/Part:BBa_K415108">BBa_K415108</a></b> | ||
| - | < | + | <ul><li> |
| - | + | Components: RBS : M13 PIII</li> | |
<li> | <li> | ||
| - | + | Explanation: This is a basic translational unit: a ribosome-binding site and the p3 gene. You can attach any promoter in front of it for expression.</li></ul> | |
<br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png"> | <br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png"> | ||
<br><br><b><a href="http://partsregistry.org/Part:BBa_K415138">BBa_K415138</a></b> | <br><br><b><a href="http://partsregistry.org/Part:BBa_K415138">BBa_K415138</a></b> | ||
| - | < | + | <ul><li> |
| - | + | Components: pLac : RBS : M13 PIII</li> | |
<li> | <li> | ||
| - | + | Explanation: This is a basic expression unit: a promoter in front of a ribosome-binding site and the p3 gene. We used this to generate constitutive expression of p3 in cells infected with hyperphage in an attempt to rescue the wild-type, non-hairy phenotype. See the results page for an AFM image of this working.</li></ul> | |
Revision as of 23:22, 24 October 2010
iGEM 2010
hairy cells and polymerizing phage - construction |
|
PARTS BBa_K415100
BBa_K415108
BBa_K415138
BBa_K415147 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-Fos Explanation: BBa_K415148 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-Jun Explanation: BBa_K415149 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-ACID Explanation: BBa_K415150 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-BASE Explanation: BBa_K415151 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-GR1 Explanation: BBa_K415152 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-GR2 Explanation: |
