Team:MIT phage construction

From 2010.igem.org

(Difference between revisions)
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<br><b><a href="http://partsregistry.org/Part:BBa_K415100">BBa_K415100</a></b>
<br><b><a href="http://partsregistry.org/Part:BBa_K415100">BBa_K415100</a></b>
<br>Components: RBS : M13 PVIII
<br>Components: RBS : M13 PVIII
-
<br>Explanation:
+
<br>Explanation: This is a basic translational unit: a ribosome-binding site and the p8 gene.  You can attach any promoter in front of it for expression.
<br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png">
<br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png">
<br><br><b><a href="http://partsregistry.org/Part:BBa_K415108">BBa_K415108</a></b>
<br><br><b><a href="http://partsregistry.org/Part:BBa_K415108">BBa_K415108</a></b>
<br>Components: RBS : M13 PIII
<br>Components: RBS : M13 PIII
-
<br>Explanation:
+
<br>Explanation: This is a basic translational unit: a ribosome-binding site and the p3 gene.  You can attach any promoter in front of it for expression.
<br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png">
<br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png">
<br><br><b><a href="http://partsregistry.org/Part:BBa_K415138">BBa_K415138</a></b>
<br><br><b><a href="http://partsregistry.org/Part:BBa_K415138">BBa_K415138</a></b>
<br>Components: pLac : RBS : M13 PIII
<br>Components: pLac : RBS : M13 PIII
-
<br>Explanation:
+
<br>Explanation: This is a basic expression unit: a promoter in front of a ribosome-binding site and the p3 gene.  We used this to generate constitutive expression of p3 in cells infected with hyperphage in an attempt to rescue the wild-type, non-hairy phenotype.  See the results page for an AFM image of this working.
<br><img src="https://static.igem.org/mediawiki/2010/4/4a/138.png">
<br><img src="https://static.igem.org/mediawiki/2010/4/4a/138.png">
<br><br><b>BBa_K415147</b>
<br><br><b>BBa_K415147</b>

Revision as of 23:18, 24 October 2010

hairy cells and polymerizing phage - construction

PARTS

BBa_K415100
Components: RBS : M13 PVIII
Explanation: This is a basic translational unit: a ribosome-binding site and the p8 gene. You can attach any promoter in front of it for expression.


BBa_K415108
Components: RBS : M13 PIII
Explanation: This is a basic translational unit: a ribosome-binding site and the p3 gene. You can attach any promoter in front of it for expression.


BBa_K415138
Components: pLac : RBS : M13 PIII
Explanation: This is a basic expression unit: a promoter in front of a ribosome-binding site and the p3 gene. We used this to generate constitutive expression of p3 in cells infected with hyperphage in an attempt to rescue the wild-type, non-hairy phenotype. See the results page for an AFM image of this working.


BBa_K415147
Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-Fos
Explanation:


BBa_K415148
Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-Jun
Explanation:


BBa_K415149
Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-ACID
Explanation:


BBa_K415150
Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-BASE
Explanation:


BBa_K415151
Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-GR1
Explanation:


BBa_K415152
Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-GR2
Explanation: