Team:MIT mge
From 2010.igem.org
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<li><a href="https://2010.igem.org/Team:MIT_mge">Genetic Engineering</a></li> | <li><a href="https://2010.igem.org/Team:MIT_mge">Genetic Engineering</a></li> | ||
<li><a href="https://2010.igem.org/Team:MIT_gateway">Gateway Cloning</a></li> | <li><a href="https://2010.igem.org/Team:MIT_gateway">Gateway Cloning</a></li> | ||
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+ | <dt><b>Phage Protocol</b></dt> | ||
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+ | <dd> | ||
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+ | <li><a href="https://2010.igem.org/Team:MIT_phageprot">Basic Protocol</a></li> | ||
</ul> | </ul> |
Latest revision as of 16:54, 27 October 2010
mammalian genetic engineering protocol |
The Mammalian team created lines of cells using lentiviruses for transfection. |
Calcium Phosphate Transfection Beforehand:
Protocol
Filter and Concentrate Virus Protocol
Materials HBSS Buffer (500 mL)
Calcium Chloride Solution (50mL)
Freezing medium
Supplemented DMEM
|