Team:MIT mge
From 2010.igem.org
(Difference between revisions)
Line 43: | Line 43: | ||
<ul id="procedure" style="list-style-type: circle;"> | <ul id="procedure" style="list-style-type: circle;"> | ||
<li>Combine CaCL2 and dilute Na2PO4 to get CaPO4 with DNA in it</li> | <li>Combine CaCL2 and dilute Na2PO4 to get CaPO4 with DNA in it</li> | ||
- | <ul>Particles must be of the right size for cells to take them up, controlled by pH: exactly 7.06</ | + | <ul><li>Particles must be of the right size for cells to take them up, controlled by pH: exactly 7.06</li> |
<li>Use each aliquot 1-2 times then throw away because air contact makes the pH drift.</li></ul> | <li>Use each aliquot 1-2 times then throw away because air contact makes the pH drift.</li></ul> | ||
<li>To make lentiviruses, need: | <li>To make lentiviruses, need: |
Revision as of 17:11, 16 October 2010
Bacterial Construction Protocol
Bacterial Experimental Protocol
Phage western blot
Mammalian Protocol
Microfluidic stress
Mammalian Protocol
Microfluidics Protocol
Genetic Engineering Protocol
mammalian microfluidic protocol |
The Mammalian team focused on creating a pressure-sensitive promoter and creating a standard protocol for mammalian genes.
1 HTD Preparation Protocol 1.1 PDMS Mixture Preparation 1.2 PDMS Pouring 1.3 PDMS Baking 1.4 PDMS-Device Punching and Bonding 1.5 PDMS Device Bonding 1.6 PDL coating 1.7 Collagen filling 1.8 Cell Seeding 2 Protocol for Deflection Experiments 2.1 Tubing Setup Details 2.2 Adding Medium to Channels 2.3 Connecting device to pressure valve 2.4 Microcontroller details |
Mammalian Genetic Engineering Protocol Calcium Phosphate Transfection
PDMS Baking
|